Journal article
Micropropagação de Pinus tecunumanii
Ciencia Florestal, Vol.28(2), p.651
2018
Abstract
Pinus tecunumanii is one of the most important tropical species of Pinus in Brazil. This work aimed to develop a micropropagation protocol for Pinus tecunumanii from the apical shoots of young plants grown in a greenhouse. To optimise the aseptic treatment protocol, the effect of 0.05 or 0.1% mercuric chloride in combination with 0 or 1 g L-1 fungicide and NaOCl was evaluated. Next, the effect of 2 or 4 µM BA (6-benzyl adenine) on in vitro multiplication was evaluated over three subcultures. Supplementation with 1.5 g L-1 activated charcoal was tested for elongation. For rooting, the effects of WV5 salts (at half strength) and 20 g L-1 sucrose were evaluated on media supplemented with 2.68 µM naphthalene-acetic acid and 0.44 µM BA. Aseptic treatment of explants with 0.05% mercuric chloride and sodium hypochlorite (with or without fungicide) was effective to establish 83.3% of explants in vitro. On media supplemented with 2 or 4 µM BA, shoot multiplication was promoted. After the third subculture on medium supplemented with 2 µM BA, 70.83% of the explants exhibited new shoots with an average of 3.7 new shoots per explant. On medium containing activated charcoal, elongation was observed in 59.76% of the explants, while on medium without activated charcoal only 33.33% elongated. Rooting was observed in 14.8% of explants on medium supplemented with WV5 salts.
Details
- Title
- Micropropagação de Pinus tecunumanii
- Authors
- L B Zanella (Author)L Franciscon (Author)Renata L Grunennvaldt (Author) - Universidade Federal do Paraná, BrazilJ De C Tomasi (Author)J Degenhardt-Goldbach (Author)
- Publication details
- Ciencia Florestal, Vol.28(2), p.651
- Publisher
- Universidade Federal de Santa Catarina - Centro de Desportos
- Date published
- 2018
- DOI
- 10.5902/1980509832058
- ISSN
- 0103-9954
- Copyright note
- Copyright © 2018 The Author. This is an open-access article distributed under the terms of the Creative Commons Attribution License
- Organisation Unit
- School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering
- Language
- English
- Record Identifier
- 99450821802621
- Output Type
- Journal article
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- Forestry
- Plant Sciences
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