Micropropagation of the Endangered Medicinal Orchid, Dendrobium officinale

Bihua Chen; Stephen J Trueman; Jianmin Li; Qianzhen Li; Huihua Fan; Juan Zhang

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Micropropagation of the Endangered Medicinal Orchid, Dendrobium officinale

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Micropropagation of the Endangered Medicinal Orchid, Dendrobium officinale

Bihua Chen, Stephen J Trueman, Jianmin Li, Qianzhen Li, Huihua Fan and Juan Zhang

Life Science Journal, Vol.11(9), pp.526-530

2014

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Abstract

Plant Biology

auxin

cytokinin

dendrobium officinale

orchid

potato extract

tissue culture

The endangered medicinal orchid, Dendrobium officinale, has low reproductive capacity in its natural environment and its wild resources are exceedingly scarce. Domestication, including development of rapid propagation methods, is needed urgently to satisfy human demand for its medicinal products. This study developed optimized micropropagation methods for D. officinale by testing the effects of tissue culture media and additives on shoot proliferation and rooting. The optimal proliferation medium for D. officinale was ½ Murashige and Skoog (MS) with 2.0 mg L-1 benzyladenine (BA) + 0.1 mg L-1 naphthaleneacetic acid (NAA) + 100 g L-1 potato extract. A rooting medium composed of ½ MS + 0.2 mg L-1 BA + 1.0 mg L-1 NAA provided 100% rooting. These methods allow reliable mass-production of D. officinale for medicinal purposes.

Details

Title: Micropropagation of the Endangered Medicinal Orchid, Dendrobium officinale
Authors: Bihua Chen (Author) - Fujian Academy of Forestry Science, China
Stephen J Trueman (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Jianmin Li (Author) - Fujian Academy of Forestry Science, China
Qianzhen Li (Author) - Fujian Academy of Forestry Science, China
Huihua Fan (Author) - Fujian Academy of Forestry Science, China
Juan Zhang (Author) - Fujian Academy of Forestry Science, China
Publication details: Life Science Journal, Vol.11(9), pp.526-530
Publisher: Zhengzhou University
Date published: 2014
ISSN: 1097-8135
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy
Language: English
Record Identifier: 99448825002621
Output Type: Journal article

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