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MicroRNA Maturation and MicroRNA Target Gene Expression Regulation Are Severely Disrupted in Soybean dicer-like1 Double Mutants2.02 MB
Published VersionCC BY V4.0, Open Access
Journal article
MicroRNA Maturation and MicroRNA Target Gene Expression Regulation Are Severely Disrupted in Soybean dicer-like1 Double Mutants
G3: Genes, Genomes, Genetics, Vol.6(2), pp.423-433
2016
PMCID: PMC4751560
PMID: 26681515
Abstract
Small nonprotein-coding microRNAs (miRNAs) are present in most eukaryotes and are central effectors of RNA silencing-mediated mechanisms for gene expression regulation. In plants, DICER-LIKE1 (DCL1) is the founding member of a highly conserved family of RNase III-like endonucleases that function as core machinery proteins to process hairpin-like precursor transcripts into mature miRNAs, small regulatory RNAs, 21-22 nucleotides in length. Zinc finger nucleases (ZFNs) were used to generate single and double-mutants of putative soybean DCL1 homologs, DCL1a and DCL1b, to confirm their functional role(s) in the soybean miRNA pathway. Neither DCL1 single mutant, dcl1a or dcl1b plants, exhibited a pronounced morphological or molecular phenotype. However, the dcl1a/dcl1b double mutant expressed a strong morphological phenotype, characterized by reduced seed size and aborted seedling development, in addition to defective miRNA precursor transcript processing efficiency and deregulated miRNA target gene expression. Together, these findings indicate that the two soybean DCL1 paralogs, DCL1a and DCL1b, largely play functionally redundant roles in the miRNA pathway and are essential for normal plant development.
Details
- Title
- MicroRNA Maturation and MicroRNA Target Gene Expression Regulation Are Severely Disrupted in Soybean dicer-like1 Double Mutants
- Authors
- Shaun J. Curtin (Author) - University of MinnesotaJean-Michel Michno (Author) - University of MinnesotaBenjamin W. Campbell (Author) - University of MinnesotaJavier Gil-Humanes (Author) - University of MinnesotaSandra M. Mathioni (Author) - University of DelawareReza Hammond (Author) - University of DelawareJuan J. Gutierrez-Gonzalez (Author) - University of MinnesotaRyan C. Donohue (Author) - University of MinnesotaMichael B. Kantar (Author) - University of MinnesotaAndrew Eamens (Author) - University of Newcastle AustraliaBlake C. Meyers (Author) - University of DelawareDaniel F. Voytas (Author) - University of MinnesotaRobert M. Stupar (Author) - University of Minnesota
- Publication details
- G3: Genes, Genomes, Genetics, Vol.6(2), pp.423-433
- Publisher
- Oxford University Press
- Date published
- 2016
- DOI
- 10.1534/g3.115.022137
- ISSN
- 2160-1836
- PMID
- 26681515; PMC4751560
- Copyright note
- This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
- Organisation Unit
- School of Health - Biomedicine; University of the Sunshine Coast, Queensland; School of Health and Behavioural Sciences - Legacy
- Language
- English
- Record Identifier
- 99656591102621
- Output Type
- Journal article
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