Methylation in the promoters of HS3ST2 and CCNA1 genes is associated with cervical cancer in Uygur women in Xinjiang

Qiangqiang Zuo; Weinan Zheng; Jinli Zhang; Zemin Pan; Yinyin Liu; Haichen Long; Peiwen Fan; Caili Guo; Feng Li; Renfu Shao

doi:10.5301/jbm.5000107

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Methylation in the promoters of HS3ST2 and CCNA1 genes is associated with cervical cancer in Uygur women in Xinjiang

Journal article

Peer reviewed

Methylation in the promoters of HS3ST2 and CCNA1 genes is associated with cervical cancer in Uygur women in Xinjiang

Qiangqiang Zuo, Weinan Zheng, Jinli Zhang, Zemin Pan, Yinyin Liu, Haichen Long, Peiwen Fan, Caili Guo, Feng Li and Renfu Shao

International Journal of Biological Markers, Vol.29(4), pp.354-362

2014

DOI: https://doi.org/10.5301/jbm.5000107

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Abstract

CIN

cervical cancer

gene methylation

HPV

We assessed the suitability of HS3ST2 and CCNA1 genes as biomarkers for the early detection of cervical cancer in Uygur women in Xinjiang, China. Methylation-specific PCR (MSP) and HPV (HPV16 and HPV18)-specific PCR were performed on 110 cervical samples: 40 normal cervices, 10 cervical intraepithelial neoplasia 1 (CIN1), 10 CIN2, 10 CIN3 and 40 cervical cancer tissues. The expression of the 2 genes was measured by reverse transcription PCR (RT-PCR) in 10 methylation-positive and 10 methylation-negative cervical tissues. We found that both HS3ST2 and CCNA1 genes were methylated in 38 of the 40 cervical cancer tissues, 9 of the 10 CIN3, and 6 of the 10 CIN2. In contrast, methylation of these 2 genes was found in only 1 of the 40 normal tissues and none of 10 CIN1. Furthermore, hypermethylated HS3ST2 and CCNA1 genes were correlated with infection with HPV16 and HPV18 in high-grade squamous intraepithelial lesions (HSILs) and cervical cancer (both p less than 0.05). The expression of HS3ST2 and CCNA1 genes was lower in the methylation-positive cervical tissues than in the methylation-negative cervical tissues. Our results indicate that HS3ST2 and CCNA1 genes may play important roles in HPV-induced cervical cancer and that patients with specific hypermethylated genes may have a greater risk of progressing to invasive cervical cancer.

Details

Title: Methylation in the promoters of HS3ST2 and CCNA1 genes is associated with cervical cancer in Uygur women in Xinjiang
Authors: Qiangqiang Zuo (Author) - Shihezi University, China
Weinan Zheng (Author) - Shihezi University, China
Jinli Zhang (Author) - Shihezi University, China
Zemin Pan (Author) - Shihezi University, China
Yinyin Liu (Author) - Shihezi University, China
Haichen Long (Author) - Shihezi University, China
Peiwen Fan (Author) - Shihezi University, China
Caili Guo (Author) - Shihezi University, China
Feng Li (Author) - Shihezi University, China
Renfu Shao (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Publication details: International Journal of Biological Markers, Vol.29(4), pp.354-362
Publisher: Wichtig Publishing
Date published: 2014
DOI: 10.5301/jbm.5000107
ISSN: 0393-6155
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99448954602621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Biotechnology & Applied Microbiology; Oncology

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