Methods of sample preparation of radula epithelial tissue in chitons (Mollusca: Polyplacophora)

Jeremy A Shaw; D J Macey; P L Clode; Lesley R Brooker; R I Webb; E J Stockdale; R M Binks

doi:10.4003/0740-2783-25.1.35

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Methods of sample preparation of radula epithelial tissue in chitons (Mollusca: Polyplacophora)

Journal article

Peer reviewed

Methods of sample preparation of radula epithelial tissue in chitons (Mollusca: Polyplacophora)

Jeremy A Shaw, D J Macey, P L Clode, Lesley R Brooker, R I Webb, E J Stockdale and R M Binks

American Malacological Bulletin, Vol.25(1), pp.35-41

2008

DOI: https://doi.org/10.4003/0740-2783-25.1.35

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Abstract

chiton

A glutaraldehyde fixative developed for preserving the radula superior epithelium of the adult chiton Acanthopleura hirtosa (Blainville, 1825), was used in conjunction with conventional and microwave-assisted sample processing to produce high quality tissue preservation for light and electron microscopy. In addition, high-pressure freezing (HPF) and cryo-substitution were used to fix the radula tissue of juvenile specimens. Microwave-assisted fixation was preferred to conventional bench-top techniques due to the superior preservation of fine cell structure together with reduced processing times and chemical exposure. Although restricted to very small (<200 μm) samples, the quality of juvenile radulae processed by HPF was excellent. The improvements in tissue preservation using microwave and cryo-preservation techniques are therefore critical for obtaining accurate ultrastructural information on the radula in marine molluscs. In particular, these findings highlight additional processing options available for the study of cellular structures in biomineralizing tissues.

Details

Title: Methods of sample preparation of radula epithelial tissue in chitons (Mollusca: Polyplacophora)
Authors: Jeremy A Shaw (Author) - Murdoch University
D J Macey (Author) - Murdoch University
P L Clode (Author) - University of Western Australia
Lesley R Brooker (Author) - University of the Sunshine Coast - Faculty of Science, Health and Education
R I Webb (Author) - University of Queensland
E J Stockdale (Author) - University of Western Australia
R M Binks (Author) - Murdoch University
Publication details: American Malacological Bulletin, Vol.25(1), pp.35-41
Publisher: American Malacological Society, Inc.
Date published: 2008
DOI: 10.4003/0740-2783-25.1.35
ISSN: 0740-2783
Organisation Unit: University of the Sunshine Coast, Queensland
Language: English
Record Identifier: 99448616502621
Output Type: Journal article

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Collaboration types: Domestic collaboration
Web Of Science research areas: Marine & Freshwater Biology; Zoology

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