Meta-Analysis of Studies Using Suppression Subtractive Hybridization and Microarrays to Investigate the Effects of Environmental Stress on Gene Transcription in Oysters

Kelli Anderson; Daisy A Taylor; Emma L Thompson; Aroon R Melwani; Sham V Nair; David A Raftos

doi:10.1371/journal.pone.0118839

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Meta-Analysis of Studies Using Suppression Subtractive Hybridization and Microarrays to Investigate the Effects of Environmental Stress on Gene Transcription in Oysters

Journal article

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Meta-Analysis of Studies Using Suppression Subtractive Hybridization and Microarrays to Investigate the Effects of Environmental Stress on Gene Transcription in Oysters

Kelli Anderson, Daisy A Taylor, Emma L Thompson, Aroon R Melwani, Sham V Nair and David A Raftos

PLoS One, Vol.10(3), e0118839

2015

DOI: https://doi.org/10.1371/journal.pone.0118839

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Abstract

Many microarray and suppression subtractive hybridization (SSH) studies have analyzed the effects of environmental stress on gene transcription in marine species. However, there have been no unifying analyses of these data to identify common stress response pathways. To address this shortfall, we conducted a meta-analysis of 14 studies that investigated the effects of different environmental stressors on gene expression in oysters. The stressors tested included chemical contamination, hypoxia and infection, as well as extremes of temperature, pH and turbidity. We found that the expression of over 400 genes in a range of oyster species changed significantly after exposure to environmental stress. A repeating pattern was evident in these transcriptional responses, regardless of the type of stress applied. Many of the genes that responded to environmental stress encoded proteins involved in translation and protein processing (including molecular chaperones), the mitochondrial electron transport chain, anti-oxidant activity and the cytoskeleton. In light of these findings, we put forward a consensus model of sub-cellular stress responses in oysters.

Details

Title: Meta-Analysis of Studies Using Suppression Subtractive Hybridization and Microarrays to Investigate the Effects of Environmental Stress on Gene Transcription in Oysters
Authors: Kelli Anderson (Author) - Macquarie University
Daisy A Taylor (Author) - Macquarie University
Emma L Thompson (Author) - Macquarie University
Aroon R Melwani (Author) - Macquarie University
Sham V Nair (Author) - Macquarie University
David A Raftos (Author) - Macquarie University
Publication details: PLoS One, Vol.10(3), e0118839; 15
Publisher: Public Library of Science
Date published: 2015
DOI: 10.1371/journal.pone.0118839
ISSN: 1932-6203
Copyright note: Copyright © 2015 Anderson et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; Centre for Bioinnovation
Language: English
Record Identifier: 99450514402621
Output Type: Journal article

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Collaboration types: Domestic collaboration
Web Of Science research areas: Marine & Freshwater Biology

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