Journal article
Mechanism of Lentinan Intestinal Absorption: Clathrin-Mediated Endocytosis and Macropinocytosis
Journal of Agricultural and Food Chemistry, Vol.69(26), pp.7344-7352
2021
PMID: 34132531
Abstract
Lentinan (LNT), a typical triple helix β-glucan extracted from Lentinus edodes, has been widely used as a functional food and an orally administered drug. However, its oral pharmacokinetics has been rarely reported. The aim of this work is to systematically study the pharmacokinetics and intestinal absorption mechanism of LNT after oral administration. Radioactive 99m-technetium (99mTc) was introduced to label LNT to determine the plasma concentration, tissue distribution, and excretion of the β-glucan in rats after oral administration. The results confirmed the absorption of LNT, with the maximal plasma concentration reached at 1 h. 5-([4,6-Dichlorotriazin-2-yl]amino)fluorescein (DTAF) was used to label LNT to explore the absorption mechanism of LNT, utilizing both a Ussing chamber and a monolayer of Caco-2 cells. These transport assays showed that LNT could penetrate through the intestine and epithelial monolayer, which was mediated by macropinocytosis and clathrin-mediated endocytosis. These findings provide a pharmacokinetic reference for LNT and help provide a greater understanding of the absorption of β-glucans in general.
Details
- Title
- Mechanism of Lentinan Intestinal Absorption: Clathrin-Mediated Endocytosis and Macropinocytosis
- Authors
- Ziming Zheng - Huazhong University of Science and TechnologyXianglin Pan - Huazhong University of Science and TechnologyHaoyu Wang - Huazhong University of Science and TechnologyZhijing Wu - Huazhong University of Science and TechnologyMitchell A Sullivan - University of QueenslandYuxuan Liu - Huazhong University of Science and TechnologyJunxi Liu - Huazhong University of Science and TechnologyKaiping Wang (Corresponding Author) - Huazhong University of Science and TechnologyYu Zhang (Corresponding Author) - Huazhong University of Science and Technology
- Publication details
- Journal of Agricultural and Food Chemistry, Vol.69(26), pp.7344-7352
- Publisher
- American Chemical Society
- Date published
- 2021
- DOI
- 10.1021/acs.jafc.1c00349
- ISSN
- 1520-5118
- PMID
- 34132531
- Organisation Unit
- School of Health - Biomedicine
- Language
- English
- Record Identifier
- 991035094602621
- Output Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web Of Science research areas
- Agriculture, Multidisciplinary
- Chemistry, Applied
- Food Science & Technology
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Source: InCites