Journal article
Measurement of tissue azithromycin levels in self-collected vaginal swabs post treatment using liquid chromatography and tandem mass spectrometry (LC-MS/MS)
PLoS One, Vol.12(5), e0177615
2017
Abstract
Background: Azithromycin is recommended for the treatment of uncomplicated urogenital chlamydia infection although the standard 1gram dose sometimes fails to eradicate the infection (treatment failure). One hypothesis proposed for treatment failure has been insufficient levels of the antibiotic at the site of infection. We developed an assay using liquid chromatography and tandem mass spectrometry (LC-MS/MS) to measure azithromycin concentration in high-vaginal swabs and monitor how concentration changes over time following routine azithromycin treatment. Methods: Azithromycin concentrations were measured in two groups of women either within the first 24h of taking a 1g dose (N = 11) or over 9 days (N = 10). Azithromycin concentrations were normalised to an internal standard (leucine enkephalin), and the bulk lipid species phosphatidylcholine [PC(34:1)], using an Agilent 6490 triple quadrupole instrument in positive ionisation mode. The abundances of azithromycin, PC(34:1), and leu-enkephalin weredetermined by multiple reaction monitoring and absolute levels of azithromycin estimated using standard curves prepared on vaginal specimens.
Details
- Title
- Measurement of tissue azithromycin levels in self-collected vaginal swabs post treatment using liquid chromatography and tandem mass spectrometry (LC-MS/MS)
- Authors
- Lenka A Vodstrcil (Author) - University of MelbourneThusitha W T Rupasinghe (Author) - University of MelbourneFabian Y S Kong (Author) - University of MelbourneDedreia Tull (Author) - University of MelbourneKaren Worthington (Author) - University of MelbourneMarcus Y Chen (Author) - University of MelbourneWilhelmina M Huston (Author) - University of Technology SydneyPeter Timms (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringMalcolm J McConville (Author) - University of MelbourneChristopher K Fairley (Author) - University of MelbourneCatriona S Bradshaw (Author) - University of MelbourneSepehr N Tabrizi (Author) - University of MelbourneJane S Hocking (Author) - University of Melbourne
- Publication details
- PLoS One, Vol.12(5), e0177615; 13
- Publisher
- Public Library of Science
- Date published
- 2017
- DOI
- 10.1371/journal.pone.0177615
- ISSN
- 1932-6203; 1932-6203
- Copyright note
- Copyright © 2017 Vodstrcil et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and eproduction in any medium, provided the original author and source are credited.
- Organisation Unit
- University of the Sunshine Coast, Queensland; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99450416102621
- Output Type
- Journal article
- Research Statement
- false
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