Measurement of tissue azithromycin levels in self-collected vaginal swabs post treatment using liquid chromatography and tandem mass spectrometry (LC-MS/MS)

Lenka A Vodstrcil; Thusitha W T Rupasinghe; Fabian Y S Kong; Dedreia Tull; Karen Worthington; Marcus Y Chen; Wilhelmina M Huston; Peter Timms; Malcolm J McConville; Christopher K Fairley; Catriona S Bradshaw; Sepehr N Tabrizi; Jane S Hocking

doi:10.1371/journal.pone.0177615

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Measurement of tissue azithromycin levels in self-collected vaginal swabs post treatment using liquid chromatography and tandem mass spectrometry (LC-MS/MS)

Journal article

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Measurement of tissue azithromycin levels in self-collected vaginal swabs post treatment using liquid chromatography and tandem mass spectrometry (LC-MS/MS)

Lenka A Vodstrcil, Thusitha W T Rupasinghe, Fabian Y S Kong, Dedreia Tull, Karen Worthington, Marcus Y Chen, Wilhelmina M Huston, Peter Timms, Malcolm J McConville, Christopher K Fairley, …

PLoS One, Vol.12(5), e0177615

2017

DOI: https://doi.org/10.1371/journal.pone.0177615

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Abstract

chlamydia infection

azithromycin treatment

azithromycin concentration

women

Background: Azithromycin is recommended for the treatment of uncomplicated urogenital chlamydia infection although the standard 1gram dose sometimes fails to eradicate the infection (treatment failure). One hypothesis proposed for treatment failure has been insufficient levels of the antibiotic at the site of infection. We developed an assay using liquid chromatography and tandem mass spectrometry (LC-MS/MS) to measure azithromycin concentration in high-vaginal swabs and monitor how concentration changes over time following routine azithromycin treatment. Methods: Azithromycin concentrations were measured in two groups of women either within the first 24h of taking a 1g dose (N = 11) or over 9 days (N = 10). Azithromycin concentrations were normalised to an internal standard (leucine enkephalin), and the bulk lipid species phosphatidylcholine [PC(34:1)], using an Agilent 6490 triple quadrupole instrument in positive ionisation mode. The abundances of azithromycin, PC(34:1), and leu-enkephalin weredetermined by multiple reaction monitoring and absolute levels of azithromycin estimated using standard curves prepared on vaginal specimens.

Details

Title: Measurement of tissue azithromycin levels in self-collected vaginal swabs post treatment using liquid chromatography and tandem mass spectrometry (LC-MS/MS)
Authors: Lenka A Vodstrcil (Author) - University of Melbourne
Thusitha W T Rupasinghe (Author) - University of Melbourne
Fabian Y S Kong (Author) - University of Melbourne
Dedreia Tull (Author) - University of Melbourne
Karen Worthington (Author) - University of Melbourne
Marcus Y Chen (Author) - University of Melbourne
Wilhelmina M Huston (Author) - University of Technology Sydney
Peter Timms (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Malcolm J McConville (Author) - University of Melbourne
Christopher K Fairley (Author) - University of Melbourne
Catriona S Bradshaw (Author) - University of Melbourne
Sepehr N Tabrizi (Author) - University of Melbourne
Jane S Hocking (Author) - University of Melbourne
Publication details: PLoS One, Vol.12(5), e0177615; 13
Publisher: Public Library of Science
Date published: 2017
DOI: 10.1371/journal.pone.0177615
ISSN: 1932-6203
Copyright note: Copyright © 2017 Vodstrcil et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and eproduction in any medium, provided the original author and source are credited.
Organisation Unit: University of the Sunshine Coast, Queensland; Centre for Bioinnovation
Language: English
Record Identifier: 99450416102621
Output Type: Journal article

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