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Published VersionCC BY V4.0, Open Access
Journal article
Maternal Diet High in Linoleic Acid Alters Renal Branching Morphogenesis and mTOR/AKT Signalling Genes in Rat Fetal Kidneys
International Journal of Molecular Sciences, Vol.25(9), pp.1-10
2024
PMID: 38731907
Abstract
Linoleic acid (LA), an n-6 polyunsaturated fatty acid (PUFA), is obtained from the maternal diet during pregnancy, and is essential for normal fetal growth and development. A maternal high-LA (HLA) diet alters maternal and offspring fatty acids, maternal leptin and male/female ratio at embryonic (E) day 20 (E20). We investigated the effects of an HLA diet on embryonic offspring renal branching morphogenesis, leptin signalling, megalin signalling and angiogenesis gene expression. Female Wistar Kyoto rats were fed low-LA (LLA; 1.44% energy from LA) or high-LA (HLA; 6.21% energy from LA) diets during pregnancy and gestation/lactation. Offspring were sacrificed and mRNA from kidneys was analysed by real-time PCR. Maternal HLA decreased the targets involved in branching morphogenesis Ret and Gdnf in offspring, independent of sex. Furthermore, downstream targets of megalin, namely mTOR, Akt3 and Prkab2, were reduced in offspring from mothers consuming an HLA diet, independent of sex. There was a trend of an increase in the branching morphogenesis target Gfra1 in females (p = 0.0517). These findings suggest that an HLA diet during pregnancy may lead to altered renal function in offspring. Future research should investigate the effects an HLA diet has on offspring kidney function in adolescence and adulthood.
Details
- Title
- Maternal Diet High in Linoleic Acid Alters Renal Branching Morphogenesis and mTOR/AKT Signalling Genes in Rat Fetal Kidneys
- Authors
- Connie McClelland - Griffith UniversityOlivia J. Holland - Griffith UniversityNirajan Shrestha - Griffith UniversityClaire L. Jukes - Griffith UniversityAnna E. Brandon - Griffith UniversityJames S. M. Cuffe - The University of QueenslandAnthony V. Perkins - University of the Sunshine Coast, Queensland, School of HealthAndrew J. McAinch - Victoria UniversityDeanne H. Hryciw (Corresponding Author) - Gold Coast Hospital
- Publication details
- International Journal of Molecular Sciences, Vol.25(9), pp.1-10
- Publisher
- MDPI AG
- Date published
- 2024
- DOI
- 10.3390/ijms25094688
- ISSN
- 1422-0067; 1661-6596
- PMID
- 38731907
- Copyright note
- © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
- Data Availability
- Data are contained within the article and Supplementary Materials.
- Organisation Unit
- School of Health - Biomedicine; School of Health
- Language
- English
- Record Identifier
- 991036485802621
- Output Type
- Journal article
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- Collaboration types
- Domestic collaboration
- Web Of Science research areas
- Biochemistry & Molecular Biology
- Chemistry, Multidisciplinary
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Source: InCites