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Marker genes of fecal indicator bacteria and potential pathogens in animal feces in a subtropical catchment919.26 kB
Accepted VersionCC BY-NC-ND V4.0, Open Access
Journal article
Marker genes of fecal indicator bacteria and potential pathogens in animal feces in a subtropical catchment
Science of the Total Environment, Vol.656, pp.1427-1435
2019
Abstract
We investigated the abundance of marker genes of two fecal indicator bacteria (FIB) and eight potential pathogens in fecal samples (n = 14) of humans and 10 domestic and native wild animals (n = 134). For each target animal, between 10 and 14 individual fecal samples were collected (n = 148 individual fecal samples in total). The abundance of FIB and potential pathogens within each sample was tested using quantitative PCR (qPCR) assays. All animals tested were positive for Escherichia coli (EC) and the concentrations ranged from 6.13 (flying fox) to 8.87 (chicken) log10 GC/g of feces. These values for Enterococcus spp. (ENT) were 5.25 log10 GC/g for flying fox and 8.12 log10 GC/g of feces for chicken. Moderate correlations were observed between EC with P. aeruginosa, EC O157 and Cryptosporidium parvum, whereas weak correlations were observed between EC and Salmonella and Giardia lamblia, Mycobacterium avium complex (MAC) and Campylobacter spp. The prevalence of MAC and P. aeruginosa were low in dog (14.3% each) and moderate (57.2%, MAC; 42.9% P. aeruginosa) in Eastern grey kangaroo fecal samples. C. parvum was detected in one cattle and one human fecal sample, while G. lamblia was detected in one dog, one flying fox, and one pig fecal samples. Among the eight potential pathogens tested, five pathogens were detected in chicken and dog fecal samples. The remaining animal species contained up to three potential pathogens in their feces. The data generated in this study will aid in the calculation of pathogen loads in the environment, and hence to assess the risks from fecal contamination of source waters.
Details
- Title
- Marker genes of fecal indicator bacteria and potential pathogens in animal feces in a subtropical catchment
- Authors
- Warish Ahmed (Author) - CSIRO Land and WaterChristian A O'Dea (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringNicole Masters (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringAnna V Kuballa (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringOswald Marinoni (Author) - CSIRO Land and WaterMohammad Katouli (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
- Publication details
- Science of the Total Environment, Vol.656, pp.1427-1435
- Publisher
- Elsevier BV
- Date published
- 2019
- DOI
- 10.1016/j.scitotenv.2018.11.439
- ISSN
- 0048-9697; 0048-9697
- Copyright note
- Copyright © 2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
- Organisation Unit
- School of Health - Biomedicine; School of Health; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy; School of Health and Sport Sciences - Legacy; Forest Industries Research Centre; School of Science, Technology and Engineering; Centre for Bioinnovation; School of Health and Behavioural Sciences - Legacy
- Language
- English
- Record Identifier
- 99451456002621
- Output Type
- Journal article
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