Journal article
Mannose-binding lectin as part of the complement pathway: Characterization in non-inflamed and inflamed human eyes
Clinical and Experimental Ophthalmology, Vol.39(9), pp.871-877
2011
PMID: 21631672
Abstract
Background: Mannose-binding lectin plays a central effector role in the lectin pathway of complement activation. Frequently occurring MBL2 polymorphisms result in mannose-binding lectin deficiency, which increases susceptibility to infection. We characterized mannose-binding lectin levels and function in non-inflamed and inflamed human eyes, and evaluated its relationship to blood mannose-binding lectin levels and function. Design: Prospective, observational clinical study with controls and cases. Participants: Twenty-seven patients with paired blood and ocular samples (aqueous and/or vitreous) including 15 controls (non-inflamed) and 12 cases (inflamed). Methods: Blood and ocular samples were collected from controls (n=15) with quiet eyes during elective cataract surgery and cases with inflamed eyes including proven/suspected endophthalmitis (n=11) and herpetic retinal vasculitis (n=1). Mannan-binding and C4 deposition enzyme-linked quantify mannose-binding lectin levels and function. Main Outcome Measures: Blood and ocular mannose-binding lectin levels and function. Results: Of 27 patients, 10 (37%) were mannose-binding lectin-deficient (defined as blood mannose-binding lectin levels <500ng/mL). Blood mannose-binding lectin levels (P= 0.16) or function (P= 0.43) were not significantly different between controls and cases. As expected, there was a high correlation between blood mannose-binding lectin levels and function (r 2= 0.74). However, there was significantly more mannose-binding lectin in inflamed eyes than non-inflamed eyes measured as level (P < 0.01) or C4 deposition function (P < 0.01). Conclusions: Our study demonstrated that mannose-binding lectin is significantly elevated in inflamed human eyes but virtually undetectable in non-inflamed control eyes, suggesting a role in sight-threatening ocular inflammation.
Details
- Title
- Mannose-binding lectin as part of the complement pathway: Characterization in non-inflamed and inflamed human eyes
- Authors
- Sing Pey Chow (Author) - The Royal Melbourne HospitalMelinda M Dean (Author) - Australian Red Cross Blood ServiceJozef A Depla (Author) - Royal Victoria Eye and Ear HospitalMark D Daniell (Author) - The Royal Melbourne HospitalDamon P Eisen (Author) - The Royal Melbourne Hospital
- Publication details
- Clinical and Experimental Ophthalmology, Vol.39(9), pp.871-877
- Date published
- 2011
- DOI
- 10.1111/j.1442-9071.2011.02572.x
- ISSN
- 1442-6404; 1442-9071; 1442-6404
- PMID
- 21631672
- Organisation Unit
- School of Health - Biomedicine; University of the Sunshine Coast, Queensland; School of Health and Behavioural Sciences - Legacy
- Language
- English
- Record Identifier
- 99513783802621
- Output Type
- Journal article
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- Domestic collaboration
- Web Of Science research areas
- Ophthalmology
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Source: InCites