MYB elongation is regulated by the nucleic acid binding of NFekB p50 to the intronic stem-loop region

Lloyd Pereira; Honor Hugo; Jordane Malaterre; Xu Huiling; Secondo Sonza; Alina Cures; Damian F J Purcell; Paul A Ramsland; Steven Gerondakis; Thomas J Gonda; Robert G Ramsay

doi:10.1371/journal.pone.0122919

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MYB elongation is regulated by the nucleic acid binding of NFekB p50 to the intronic stem-loop region

Journal article

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Peer reviewed

MYB elongation is regulated by the nucleic acid binding of NFekB p50 to the intronic stem-loop region

Lloyd Pereira, Honor Hugo, Jordane Malaterre, Xu Huiling, Secondo Sonza, Alina Cures, Damian F J Purcell, Paul A Ramsland, Steven Gerondakis, Thomas J Gonda, …

PLoS One, Vol.10(4), pp.1-25

2015

DOI: https://doi.org/10.1371/journal.pone.0122919

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Abstract

protein Myb

MYB transcriptional elongation is regulated by an attenuator sequence within intron 1 that has been proposed to encode a RNA stem loop (SLR) followed by a polyU tract. We report that NFekBp50 can bind the SLR polyU RNA and promote MYB transcriptional elongation together with NFekBp65. We identified a conserved lysine-rich motif within the Rel homology domain (RHD) of NFekBp50, mutation of which abrogated the interaction of NFekBp50 with the SLR polyU and impaired NFekBp50 mediated MYB elongation. We observed that the TAR RNA-binding region of Tat is homologous to the NFekBp50 RHD lysine-rich motif, a finding consistent with HIV Tat acting as an effector of MYB transcriptional elongation in an SLR dependent manner. Furthermore, we identify the DNA binding activity of NFekBp50 as a key component required for the SLR polyU mediated regulation of MYB. Collectively these results suggest that the MYB SLR polyU provides a platform for proteins to regulate MYB and reveals novel nucleic acid binding properties of NFekBp50 required for MYB regulation. Refereed/Peer-reviewed

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Title: MYB elongation is regulated by the nucleic acid binding of NFekB p50 to the intronic stem-loop region
Authors: Lloyd Pereira (Author) - The University of Melbourne
Honor Hugo (Author) - St Vincents Institute of Medical Research
Jordane Malaterre (Author) - The University of Melbourne
Xu Huiling (Author) - The University of Melbourne
Secondo Sonza (Author) - The University of Melbourne
Alina Cures (Author) - Peter MacCallum Cancer Centre
Damian F J Purcell (Author) - The University of Melbourne
Paul A Ramsland (Author) - Burnet Institute
Steven Gerondakis (Author) - Monash University
Thomas J Gonda (Author) - The University of Queensland
Robert G Ramsay (Author) - The University of Melbourne
Publication details: PLoS One, Vol.10(4), pp.1-25
Publisher: Public Library of Science
Date published: 2015
DOI: 10.1371/journal.pone.0122919
ISSN: 1932-6203
Copyright note: © 2015 Pereira et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Organisation Unit: University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; School of Health and Behavioural Sciences - Legacy
Language: English
Record Identifier: 99518707102621
Output Type: Journal article

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Web Of Science research areas: Biochemistry & Molecular Biology

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