MRI changes and complement activation correlate with epileptogenicity in a mouse model of temporal lobe epilepsy

I Kharatishvili; Zack Y Shan; D T She; S Foong; N D Kurniawan; D C Reutens

doi:10.1007/s00429-013-0528-4

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MRI changes and complement activation correlate with epileptogenicity in a mouse model of temporal lobe epilepsy

Journal article

Peer reviewed

MRI changes and complement activation correlate with epileptogenicity in a mouse model of temporal lobe epilepsy

I Kharatishvili, Zack Y Shan, D T She, S Foong, N D Kurniawan and D C Reutens

Brain Structure and Function, Vol.219(2), pp.683-706

2014

DOI: https://doi.org/10.1007/s00429-013-0528-4

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Abstract

epileptogenesis

spontaneous recurrent seizures

temporal lobe epilepsy

magnetic resonance imaging

complement

hippocampus

parahippocampal cortex

epilepsy animal models

The complex pathogenesis of temporal lobe epilepsy includes neuronal and glial pathology, synaptic reorganization, and an immune response. However, the spatio-temporal pattern of structural changes in the brain that provide a substrate for seizure generation and modulate the seizure phenotype is yet to be completely elucidated. We used quantitative magnetic resonance imaging (MRI) to study structural changes triggered by status epilepticus (SE) and their association with epileptogenesis and with activation of complement component 3 (C3). SE was induced by injection of pilocarpine in CD1 mice. Quantitative diffusion-weighted imaging and T2 relaxometry was performed using a 16.4-Tesla MRI scanner at 3 h and 1, 2, 7, 14, 28, 35, and 49 days post-SE. Following longitudinal MRI examinations, spontaneous recurrent seizures and interictal spikes were quantified using continuous video-EEG monitoring. Immunohistochemical analysis of C3 expression was performed at 48 h, 7 days, and 4 months post-SE. MRI changes were dynamic, reflecting different outcomes in relation to the development of epilepsy. Apparent diffusion coefficient changes in the hippocampus at 7 days post-SE correlated with the severity of the evolving epilepsy. C3 activation was found in all stages of epileptogenesis within the areas with significant MRI changes and correlated with the severity of epileptic condition.

Details

Title: MRI changes and complement activation correlate with epileptogenicity in a mouse model of temporal lobe epilepsy
Authors: I Kharatishvili (Author) - University of Queensland
Zack Y Shan (Author) - University of Queensland
D T She (Author) - University of Queensland
S Foong (Author) - University of Queensland
N D Kurniawan (Author) - University of Queensland
D C Reutens (Author) - University of Queensland
Publication details: Brain Structure and Function, Vol.219(2), pp.683-706
Publisher: Springer
Date published: 2014
DOI: 10.1007/s00429-013-0528-4
ISSN: 1863-2653
Organisation Unit: University of the Sunshine Coast, Queensland; Thompson Institute
Language: English
Record Identifier: 99451002802621
Output Type: Journal article

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