Low temperature alters plasma membrane lipid composition and ATPase activity of pineapple fruit during blackheart development

Yuchan Zhou; X Pan; H Qu; Steven J R Underhill

doi:10.1007/s10863-013-9538-4

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Low temperature alters plasma membrane lipid composition and ATPase activity of pineapple fruit during blackheart development

Journal article

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Low temperature alters plasma membrane lipid composition and ATPase activity of pineapple fruit during blackheart development

Yuchan Zhou, X Pan, H Qu and Steven J R Underhill

Journal of Bioenergetics and Biomembranes, Vol.46(1), pp.59-69

2014

DOI: https://doi.org/10.1007/s10863-013-9538-4

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Abstract

plasma membrane

ATPase

lipid composition

blackheart (chilling injury)

pineapple fruit

Plasma membrane (PM) plays central role in triggering primary responses to chilling injury and sustaining cellular homeostasis. Characterising response of membrane lipids to low temperature can provide important information for identifying early causal factors contributing to chilling injury. To this end, PM lipid composition and ATPase activity were assessed in pineapple fruit (Ananas comosus) in relation to the effect of low temperature on the development of blackheart, a form of chilling injury. Chilling temperature at 10 °C induced blackheart development in concurrence with increase in electrolyte leakage. PM ATPase activity was decreased after 1 week at low temperature, followed by a further decrease after 2 weeks. The enzyme activity was not changed during 25 °C storage. Loss of total PM phospholipids was found during postharvest senescence, but more reduction was shown from storage at 10 °C. Phosphatidylcholine and phosphatidylethanolamine were the predominant PM phospholipid species. Low temperature increased the level of phosphatidic acid but decreased the level of phosphatidylinositol. Both phospholipid species were not changed during storage at 25 °C. Postharvest storage at both temperatures decreased the levels of C18:3 and C16:1, and increased level of C18:1. Low temperature decreased the level of C18:2 and increased the level of C14:0. Exogenous application of phosphatidic acid was found to inhibit the PM ATPase activity of pineapple fruit in vitro. Modification of membrane lipid composition and its effect on the functional property of plasma membrane at low temperature were discussed in correlation with their roles in blackheart development of pineapple fruit.

Details

Title: Low temperature alters plasma membrane lipid composition and ATPase activity of pineapple fruit during blackheart development
Authors: Yuchan Zhou (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
X Pan (Author) - Chinese Academy of Sciences, China
H Qu (Author) - Chinese Academy of Sciences, China
Steven J R Underhill (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Publication details: Journal of Bioenergetics and Biomembranes, Vol.46(1), pp.59-69
Publisher: Springer New York LLC
Date published: 2014
DOI: 10.1007/s10863-013-9538-4
ISSN: 0145-479X
Organisation Unit: Australian Centre for Pacific Islands Research; University of the Sunshine Coast, Queensland; Sustainability Research Cluster
Language: English
Record Identifier: 99448975802621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Biophysics; Cell Biology