Location and post-mortem changes in some cytoskeletal proteins in pork and cod muscle

E H Morrison; H A Bremner; P P Purslow

doi:10.1002/(SICI)1097-0010(20000501)80:6<691::AID-JSFA590>3.0.CO;2-T

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Location and post-mortem changes in some cytoskeletal proteins in pork and cod muscle

Journal article

Peer reviewed

Location and post-mortem changes in some cytoskeletal proteins in pork and cod muscle

E H Morrison, H A Bremner and P P Purslow

Journal of the Science of Food and Agriculture, Vol.80(7), pp.691-697

2000

DOI: https://doi.org/10.1002/(SICI)1097-0010(20000501)80:6<691::AID-JSFA590>3.0.CO;2-T

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https://doi.org/10.1002/(SICI)1097-0010(20000501)80:6<691::AID-JSFA590>3.0.CO;2-TView

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Abstract

Agricultural and Veterinary Sciences

Engineering

cytoskeleton

muscle

intermediate filaments

pork

fish

cod

actin

desmin

talin

vinculin

nebulin

spectrin

post-mortem changes

ultrastructure

The cytoskeletal proteins actin, nebulin, spectrin, desmin, vinculin and talin were labelled immunohistochemically in sections of muscle from commercially available pigs and cod (Gadus morhua ) taken pre‐rigor and from samples stored for several days. Actin, nebulin and spectrin gave similar labelling patterns in both pork and cod muscle which remained the same in stored samples. Desmin was intensely labelled at the cell boundaries and within the body of the cells in both pork and cod in the initial and the stored samples. Vinculin was readily labelled in pork muscle but showed only diffuse labelling in fish. Labelling for talin in pork muscle was intense at the sarcolemma but was not present in samples stored for 4 days. In contrast, the label for talin was concentrated at the myotendinous junction of the cod muscle throughout the storage period. These are the first reports of the detection and location of spectrin and vinculin in fish muscle and of the location of talin. The results are discussed in terms of muscle structure, function and post‐mortem tenderisation.

Details

Title: Location and post-mortem changes in some cytoskeletal proteins in pork and cod muscle
Authors: E H Morrison (Author) - Royal Danish Agricultural and Veterinary University, Denmark
H A Bremner (Author) - Danish Institute of Fisheries Research, Denmark
P P Purslow (Author) - Royal Danish Agricultural and Veterinary University, Denmark
Publication details: Journal of the Science of Food and Agriculture, Vol.80(7), pp.691-697
Publisher: John Wiley & Sons Ltd.
Date published: 2000
DOI: 10.1002/(SICI)1097-0010(20000501)80:6<691::AID-JSFA590>3.0.CO;2-T
ISSN: 0022-5142
Organisation Unit: University of the Sunshine Coast, Queensland
Language: English
Record Identifier: 99450143502621
Output Type: Journal article

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Collaboration types: Domestic collaboration
Web Of Science research areas: Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology