Localization of plasma membrane and secretory calcium pumps in the mammary gland

Helen Faddy; Chanel E Smart; Ren Xu; Genee Y Lee; Paraic A Kenny; Mingye Feng; Rajini Rao; Melissa A Brown; Mina J Bissell; Sarah J Roberts-Thomson; Gregory R Monteith

doi:10.1016/j.bbrc.2008.03.003

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Localization of plasma membrane and secretory calcium pumps in the mammary gland

Journal article

Peer reviewed

Localization of plasma membrane and secretory calcium pumps in the mammary gland

Helen Faddy, Chanel E Smart, Ren Xu, Genee Y Lee, Paraic A Kenny, Mingye Feng, Rajini Rao, Melissa A Brown, Mina J Bissell, Sarah J Roberts-Thomson, …

Biochemical and Biophysical Research Communications, Vol.369(3), pp.977-981

2008

DOI: https://doi.org/10.1016/j.bbrc.2008.03.003

PMCID: PMC3234104

PMID: 18334228

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Abstract

SPCA2

SPCA1

3D mammary culture

Calcium

Mouse

Mammary gland

PMCA2

PMCA1

Milk

Until recently the mechanism for the enrichment of milk with calcium was thought to be almost entirely via the secretory pathway. However, recent studies suggest that a plasma membrane calcium ATPase, PMCA2, is the primary mechanism for calcium transport into milk, highlighting a major role for apical calcium transport. We compared the expression of the recently identified secretory calcium ATPase, SPCA2, and SPCA1, in the mouse mammary gland during development. SPCA2 levels increased over 35-fold during lactation with expression localized to luminal secretory cells, while SPCA1 increased only a modest 2-fold and was expressed throughout the cells of the mammary gland. We also observed major differences in the localization of PMCA2 and PMCA1. Our studies highlight the likely specific roles of PMCA2 and SPCA2 in lactation and indicate that calcium transport into milk is a complex interplay between apical and secretory pathways.

Details

Title: Localization of plasma membrane and secretory calcium pumps in the mammary gland
Authors: Helen Faddy (Author) - University of Queensland
Chanel E Smart (Author) - University of Queensland
Ren Xu (Author) - University of California, Berkeley
Genee Y Lee (Author) - University of California, Berkeley
Paraic A Kenny (Author) - University of California, Berkeley
Mingye Feng (Author) - Johns Hopkins University
Rajini Rao (Author) - Johns Hopkins University
Melissa A Brown (Author) - University of Queensland
Mina J Bissell (Author) - University of California, Berkeley
Sarah J Roberts-Thomson (Author) - University of Queensland
Gregory R Monteith (Author) - University of Queensland
Publication details: Biochemical and Biophysical Research Communications, Vol.369(3), pp.977-981
Publisher: Elsevier Inc.
DOI: 10.1016/j.bbrc.2008.03.003
ISSN: 1090-2104
PMID: 18334228; PMC3234104
Organisation Unit: School of Health and Behavioural Sciences - Legacy; School of Health and Sport Sciences - Legacy; University of the Sunshine Coast, Queensland; School of Health - Biomedicine
Language: English
Record Identifier: 99477806102621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Biochemistry & Molecular Biology; Biophysics

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