Liver glycogen fragility in the presence of hydrogen-bond breakers

Xinle Tan; Ziyi Wang; Ut Cheung; Zhenxia Hu; Qinghua Liu; Liang Wang; Mitchell A Sullivan; Daniel Cozzolino; Robert G Gilbert

doi:10.1016/j.ijbiomac.2024.131741

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Liver glycogen fragility in the presence of hydrogen-bond breakers

Journal article

Open access

Peer reviewed

Liver glycogen fragility in the presence of hydrogen-bond breakers

Xinle Tan, Ziyi Wang, Ut Cheung, Zhenxia Hu, Qinghua Liu, Liang Wang, Mitchell A Sullivan, Daniel Cozzolino and Robert G Gilbert

International Journal of Biological Macromolecules, Vol.268(Pt 1), pp.1-8

2024

DOI: https://doi.org/10.1016/j.ijbiomac.2024.131741

PMID: 38649083

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Abstract

Diabetes

Glycogen

Hydrogen-bond breaker

Molecular structure

Mouse model

Glycogen, a complex branched glucose polymer, is responsible for sugar storage in blood glucose homeostasis. It comprises small β particles bound together into composite α particles. In diabetic livers, α particles are fragile, breaking apart into smaller particles in dimethyl sulfoxide, DMSO; they are however stable in glycogen from healthy animals. We postulate that the bond between β particles in α particles involves hydrogen bonding. Liver-glycogen fragility in normal and db/db mice (an animal model for diabetes) is compared using various hydrogen-bond breakers (DMSO, guanidine and urea) at different temperatures. The results showed different degrees of α-particle disruption. Disrupted glycogen showed changes in the mid-infra-red spectrum that are related to hydrogen bonds. While glycogen α-particles are only fragile under harsh, non-physiological conditions, these results nevertheless imply that the bonding between β particles in α particles is different in diabetic livers compared to healthy, and is probably associated with hydrogen bonding.

Details

Title: Liver glycogen fragility in the presence of hydrogen-bond breakers
Authors: Xinle Tan - The University of Queensland
Ziyi Wang - The University of Queensland
Ut Cheung - The University of Queensland
Zhenxia Hu - Fudan University
Qinghua Liu - Macau University of Science and Technology
Liang Wang - Xuzhou Medical College
Mitchell A Sullivan - The University of Queensland
Daniel Cozzolino - The University of Queensland
Robert G Gilbert (Corresponding Author) - The University of Queensland
Publication details: International Journal of Biological Macromolecules, Vol.268(Pt 1), pp.1-8
Publisher: Elsevier BV
Date published: 2024
DOI: 10.1016/j.ijbiomac.2024.131741
ISSN: 1879-0003; 0141-8130
PMID: 38649083
Data Availability: Data will be made available on request.
Organisation Unit: School of Health - Biomedicine
Language: English
Record Identifier: 991035092502621
Output Type: Journal article

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