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Journal article
Limosilactobacillus reuteri M4-100 Mitigates the Pathogenicity of Escherichia coli Strain HMLN-1 in an Intestinal Epithelial Model and Modulates Host Cell Gene Expression
Microorganisms, Vol.13(6), pp.1-16
2025
PMID: 40572318
Abstract
Probiotics have been widely adopted due to their beneficial health properties. Here, we investigated the interactions of a probiotic Limosilactobacillus (Lactobacillus) reuteri M4-100, with a translocating Escherichia coli strain HMLN-1, in a co-culture of cells, representing the intestinal epithelium, and identified molecular mechanisms associated with the host response. A co-culture of Caco-2:HT29-MTX cells was exposed to the HMLN-1 strain and the route of translocation was studied. Scanning and transmission electron microscopy revealed the adhesion of the strain to the microvilli, the establishment of close contact with the co-culture prior to being taken up by membrane-bound vesicles, and translocation via the intracellular pathway. When the HMLN-1 strain was challenged with L. reuteri M4-100 in co- and pre-inoculation experiments, its adhesion to the co-culture of cells was significantly reduced (p < 0.0001). A significant reduction in the invasion of the HMLN-1 strain was also observed upon the inoculation of L. reuteri M4-100 with the co-culture 60 min prior to HMLN-1 exposure (p < 0.0001). The L. reuteri M4-100 strain also significantly (p < 0.0001) reduced the translocation of the HMLN-1 strain in both co- and pre-inoculation experiments. Differential gene expression studies identified key cellular responses to the interaction with these bacteria, both alone. These data demonstrate the efficacy of L. reuteri M4-100 to reduce or inhibit the interaction of E. coli HMLN-1 with the intestinal epithelium. A prophylactic role of this probiotic strain is postulated as these effects were more pronounced in pre-inoculation experiments.
Details
- Title
- Limosilactobacillus reuteri M4-100 Mitigates the Pathogenicity of Escherichia coli Strain HMLN-1 in an Intestinal Epithelial Model and Modulates Host Cell Gene Expression
- Authors
- Behnoush Asgari - University of the Sunshine Coast, Queensland, Centre for BioinnovationGeorgia Bradford - University of the Sunshine Coast, Queensland, Centre for BioinnovationEva Hatje - Queensland University of TechnologyAnna Kuballa - University of the Sunshine Coast, Queensland, Centre for BioinnovationMohammad Katouli (Corresponding Author) - University of the Sunshine Coast, Queensland, Centre for Bioinnovation
- Publication details
- Microorganisms, Vol.13(6), pp.1-16
- Publisher
- MDPI AG
- Date published
- 2025
- DOI
- 10.3390/microorganisms13061428
- ISSN
- 2076-2607
- PMID
- 40572318
- Copyright note
- © 2025 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
- Data Availability
- The original contributions presented in this study are included in the article. Further inquiries can be directed to the corresponding author.
- Grant note
- This research was funded by the Research Training Program (RTP) scholarship provided To B. Asgari by the University of the Sunshine Coast and the Australian Commonwealth Government.
- Organisation Unit
- School of Health - Biomedicine; School of Science and Engineering - Legacy; School of Health and Sport Sciences - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 991136273602621
- Output Type
- Journal article