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Journal article
Limited potential of sheep as a large animal to model transfusion-related acute lung injury mediated by antibodies against human neutrophil antigen 3a
Annals of Blood, Vol.9, pp.1-9
2024
Abstract
Transfusion-related acute lung injury (TRALI) is a potentially life-threatening adverse transfusion reaction. Many severe cases of TRALI relate to transfusion of antibodies against human neutrophil antigen 3 (HNA-3). Characterization of the HNA-3 antigen on choline transporter-like protein 2 (CTL2) led to the discovery that human anti-HNA-3a alloantibodies show some cross-reactivity with mouse CTL2. This suggested that human anti-HNA-3a antibodies may react with CTL2 from other species. Although small rodent models are crucial for scientific research, their clinical relevance may be limited due to differences in physiology and, as a result, findings obtained from murine models may not be directly applicable to clinical settings. Sheep have previously been used to model non-antibody mediated TRALI and were therefore assessed for their potential to model for anti-HNA-3a mediated TRALI. Sequences for CTL2 and the gene that encodes it (SLC44A2) from humans, mice, and sheep were compared using publically available databases. Binding experiments were performed with human sera containing an anti-HNA-3a antibody and human, mouse and sheep cells. Although analysis showed that sheep SLC44A2 and CTL2 shared high homology with both human and mouse counterparts (88–89% gene homology, 95–96% protein homology), including the Arg154 residue responsible the HNA-3a variant, no significant anti-HNA-3a alloantibody binding was observed with sheep cells. Therefore, this study suggests that sheep would not be a suitable large animal in which to model TRALI mediated by human anti-HNA-3a antibodies.
Details
- Title
- Limited potential of sheep as a large animal to model transfusion-related acute lung injury mediated by antibodies against human neutrophil antigen 3a
- Authors
- Filip Radenkovic (Corresponding Author) - The University of QueenslandMark Burton - Australian Red Cross LifebloodPenny Hassell - Australian Red Cross LifebloodSara Chiaretti - Australian Red Cross LifebloodXuan Bui - Australian Red Cross Blood ServiceShen Zhao - The University of QueenslandMartina Jones - The University of QueenslandRobert Flower - Australian Red Cross LifebloodJohn-Paul Tung - University of the Sunshine Coast, Queensland, School of Health and Behavioural Sciences - Legacy
- Publication details
- Annals of Blood, Vol.9, pp.1-9
- Publisher
- AME Publishing Company
- Date published
- 2024
- DOI
- 10.21037/aob-23-57
- ISSN
- 2521-361X
- Copyright note
- This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0/.
- Grant note
- This project utilised infrastructure provided by the National Biologics Facility, an initiative of the Australian Government being conducted as part of the National Collaborative Research Infrastructure Strategy. Australian Governments fund the Australian Red Cross Lifeblood for the provision of blood, blood products and services to the Australian Community.
- Organisation Unit
- School of Health; School of Health and Behavioural Sciences - Legacy
- Language
- English
- Record Identifier
- 991076696902621
- Output Type
- Journal article
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