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Knockout of glutathione peroxidase 5 down-regulates the piRNAs in the caput epididymidis of aged mice7.94 MB
Published VersionCC BY V4.0, Open Access
Journal article
Knockout of glutathione peroxidase 5 down-regulates the piRNAs in the caput epididymidis of aged mice
Asian Journal of Andrology, Vol.22(6), pp.590-601
2020
PMID: 32270769
Abstract
The mammalian epididymis not only plays a fundamental role in the maturation of spermatozoa, but also provides protection against various stressors. The foremost among these is the threat posed by oxidative stress, which arises from an imbalance in reactive oxygen species and can elicit damage to cellular lipids, proteins, and nucleic acids. In mice, the risk of oxidative damage to spermatozoa is mitigated through the expression and secretion of glutathione peroxidase 5 (GPX5) as a major luminal scavenger in the proximal caput epididymidal segment. Accordingly, the loss of GPX5-mediated protection leads to impaired DNA integrity in the spermatozoa of aged Gpx5(-/-) mice. To explore the underlying mechanism, we have conducted transcriptomic analysis of caput epididymidal epithelial cells from aged (13 months old) Gpx5(-/-) mice. This analysis revealed the dysregulation of several thousand epididymal mRNA transcripts, including the downregulation of a subgroup of piRNA pathway genes, in aged Gpx5(-/-) mice. In agreement with these findings, we also observed the loss of piRNAs, which potentially bind to the P-element-induced wimpy testis (PIWI)-like proteins PIWIL1 and PIWIL2. The absence of these piRNAs was correlated with the elevated mRNA levels of their putative gene targets in the caput epididymidis of Gpx5(-/-) mice. Importantly, the oxidative stress response genes tend to have more targeting piRNAs, and many of them were among the top increased genes upon the loss of GPX5. Taken together, our findings suggest the existence of a previously uncharacterized somatic piRNA pathway in the mammalian epididymis and its possible involvement in the aging and oxidative stress-mediated responses.
Details
- Title
- Knockout of glutathione peroxidase 5 down-regulates the piRNAs in the caput epididymidis of aged mice
- Authors
- Chen Chu (Author) - University of Chinese Academy of SciencesL Yu (Author) - University of Chinese Academy of SciencesJoelle Henry-Berger (Author) - Université Clermont AuvergneYan-Fei Ru (Author) - University of Chinese Academy of SciencesAyhan Kocer (Author) - Université Clermont AuvergneAlexandre Champroux (Author) - Université Clermont AuvergneZhi-Tong Li (Author) - University of Chinese Academy of SciencesMiao He (Author) - University of Chinese Academy of SciencesSheng-Song Xie (Author) - Huazhong Agricultural UniversityWu-Bin Ma (Author) - University of Chinese Academy of SciencesMin-Jie Ni (Author) - University of Chinese Academy of SciencesZi-Mei Ni (Author) - University of Chinese Academy of SciencesYun-Li Guo (Author) - University of Chinese Academy of SciencesZhao-Liang Fei (Author) - University of Chinese Academy of SciencesLan-Tao Gou (Author) - University of California, San DiegoQiang Liu (Author) - Shanghai Jiao Tong UniversitySanjay Sharma (Author) - Harvard Medical SchoolYu Zhou (Author) - Harvard Medical SchoolMo-Fang Liu (Author) - University of Chinese Academy of SciencesCharlie Degui Chen (Author) - University of Chinese Academy of SciencesAndrew Eamens (Author) - University of Newcastle AustraliaBrett Nixon (Author) - University of Newcastle AustraliaYu-Chuan Zhou (Author) - University of Chinese Academy of SciencesJoel R. Drevet (Author) - Université Clermont AuvergneYong Zhang (Author) - University of Chinese Academy of Sciences
- Publication details
- Asian Journal of Andrology, Vol.22(6), pp.590-601
- Publisher
- Wolters Kluwer - Medknow Publications and Media Pvt. Ltd.
- Date published
- 2020
- DOI
- 10.4103/aja.aja_3_20
- ISSN
- 1745-7262; 1008-682X
- PMID
- 32270769
- Copyright note
- (c) The Author(s)(2020). his is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
- Organisation Unit
- School of Health - Biomedicine; University of the Sunshine Coast, Queensland; School of Health and Behavioural Sciences - Legacy
- Language
- English
- Record Identifier
- 99656592802621
- Output Type
- Journal article
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- Urology & Nephrology
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