Kinematics of intracellular chlamydiae provide evidence for contact-dependent development

D P Wilson; J A Whittum-Hudson; Peter Timms; P M Bavoil

doi:10.1128/JB.00293-09

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Kinematics of intracellular chlamydiae provide evidence for contact-dependent development

Journal article

Open access

Peer reviewed

Kinematics of intracellular chlamydiae provide evidence for contact-dependent development

D P Wilson, J A Whittum-Hudson, Peter Timms and P M Bavoil

Journal of Bacteriology, Vol.191(18), pp.5734-5742

2009

DOI: https://doi.org/10.1128/JB.00293-09

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Abstract

intracellular chlamydiae

A crucial process of chlamydial development involves differentiation of the replicative reticulate body (RB) into the infectious elementary body (EB). We present experimental evidence to provide support for a contact-dependent hypothesis for explaining the trigger involved in differentiation. We recorded live-imaging of Chlamydia trachomatis-infected McCoy cells at key times during development and tracked the temporospatial trajectories of individual chlamydial particles. We found that movement of the particles is related to development. Early to mid-developmental stages involved slight wobbling of RBs. The average speed of particles increased sharply at 24 h postinfection (after the estimated onset of RB to EB differentiation). We also investigated a penicillin- supplemented culture containing EBs, RBs, and aberrantly enlarged, stressed chlamydiae. Near-immobile enlarged particles are consistent with their continued tethering to the chlamydial inclusion membrane (CIM). We found a significantly negative, nonlinear association between speed and size/type of particles, providing further support for the hypothesis that particles become untethered near the onset of RB to EB differentiation. This study establishes the relationship between the motion properties of the chlamydiae and developmental stages, whereby wobbling RBs gradually lose contact with the CIM, and RB detachment from the CIM is coincidental with the onset of late differentiation.

Details

Title: Kinematics of intracellular chlamydiae provide evidence for contact-dependent development
Authors: D P Wilson (Author) - University of New South Wales
J A Whittum-Hudson (Author) - Wayne State University School of Medicine, United States
Peter Timms (Author) - Queensland University of Technology
P M Bavoil (Author) - University of Maryland, United States
Publication details: Journal of Bacteriology, Vol.191(18), pp.5734-5742
Publisher: American Society for Microbiology
Date published: 2009
DOI: 10.1128/JB.00293-09
ISSN: 0021-9193
Copyright note: Copyright © 2009 American Society for Microbiology. The author's accepted version is reproduced here in accordance with the publisher's copyright policy. The definitive version is available at http://aem.asm.org/
Organisation Unit: University of the Sunshine Coast, Queensland; Centre for Bioinnovation
Language: English
Record Identifier: 99448621002621
Output Type: Journal article

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Web Of Science research areas: Microbiology

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