Isolation of Organic Matrix Nacreous Proteins from Haliotis diversicolor and Their Effect On In Vitro Osteoinduction

Supita Tanasawet; Boonsirm Withyachumnarnkul; Chinarat Changsangfar; Scott F Cummins; Morokot Sroyraya; Parisatcha Sangsuwan; Yindee Kitiyanant; Somluck Asuvapongpatana; Wattana Weerachatyanukul

doi:10.4002/040.056.0208

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Isolation of Organic Matrix Nacreous Proteins from Haliotis diversicolor and Their Effect On In Vitro Osteoinduction

Journal article

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Isolation of Organic Matrix Nacreous Proteins from Haliotis diversicolor and Their Effect On In Vitro Osteoinduction

Supita Tanasawet, Boonsirm Withyachumnarnkul, Chinarat Changsangfar, Scott F Cummins, Morokot Sroyraya, Parisatcha Sangsuwan, Yindee Kitiyanant, Somluck Asuvapongpatana and Wattana Weerachatyanukul

Malacologia, Vol.56(1&2), pp.107-119

2013

DOI: https://doi.org/10.4002/040.056.0208

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Abstract

haliotis diversicolor

nacre

bone

cell differentiation

osteogenesis

The organic matrix proteins of molluscan nacre are known to engage biocompatible and osteoinductive properties. In this study, we aimed to extract Haliotis diversicolor nacreous proteins and assayed their effect on pre-osteoblastic cell differentiation. We found that acid extracted nacreous proteins (AEP) consisted primarily of a major protein band of 25 kDa and three other minor proteins. AEP enhanced alkaline phosphatase (ALP) activity of MC3T3-E1 cells both in time- and concentration-dependent manners. Transcriptional up-regulation of osteogenic markers, including collagen type I (COL-I), osteopontin (OPN) and osteocalcin (OCN), was also apparent in days 7 and 14 upon AEP treatments. At the translational level, higher protein expression of COL-I was evident in AEP treated cells, and the protein was presumably laid down as extracellular matrix. Further de novo sequencing of an AEP major protein revealed a match with the abalone mantle protein sometsuke. Conclusively, we demonstrated that H. diversicolor AEP contains a factor, potentially the mantle protein sometsuke, which may impart in the osteoblastic cell differentiation.

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Title: Isolation of Organic Matrix Nacreous Proteins from Haliotis diversicolor and Their Effect On In Vitro Osteoinduction
Authors: Supita Tanasawet (Author) - Mahidol University, Thailand
Boonsirm Withyachumnarnkul (Author) - Mahidol University, Thailand
Chinarat Changsangfar (Author) - Mahidol University, Thailand
Scott F Cummins (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Morokot Sroyraya (Author) - Mahidol University, Thailand
Parisatcha Sangsuwan (Author) - Mahidol University, Thailand
Yindee Kitiyanant (Author) - Mahidol University, Thailand
Somluck Asuvapongpatana (Author) - Mahidol University, Thailand
Wattana Weerachatyanukul (Author) - Mahidol University, Thailand
Publication details: Malacologia, Vol.56(1&2), pp.107-119
Publisher: Institute of Malacology
Date published: 2013
DOI: 10.4002/040.056.0208
ISSN: 0076-2997
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99448982402621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Zoology

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