Isolation and characterization of the complete cDNA sequence encoding a putative insulin-like peptide from the androgenic gland of Penaeus monodon

Vijay R Mareddy; O Rosen; H Thaggard; R Manor; Anna V Kuballa; E D Aflalo; A Sagi; B Paterson; Abigail Elizur

doi:10.1016/j.aquaculture.2011.05.027

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Isolation and characterization of the complete cDNA sequence encoding a putative insulin-like peptide from the androgenic gland of Penaeus monodon

Journal article

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Isolation and characterization of the complete cDNA sequence encoding a putative insulin-like peptide from the androgenic gland of Penaeus monodon

Vijay R Mareddy, O Rosen, H Thaggard, R Manor, Anna V Kuballa, E D Aflalo, A Sagi, B Paterson and Abigail Elizur

Aquaculture, Vol.318(3-4), pp.364-370

2011

DOI: https://doi.org/10.1016/j.aquaculture.2011.05.027

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Abstract

androgenic gland

insulin-like gene

Penaeus monodon

monosex aquaculture

Sexual differentiation in male crustaceans is known to be controlled by the androgenic gland (AG), possibly through a peptide hormone. Recently, three freshwater and one marine crustacean decapod genes encoding AG-specific insulin-like peptides were characterized. We report here the molecular cloning of the complete sequence encoding an AG-specific insulin-like peptide (Pm-IAG) in the commercially important marine Penaeid prawn, Penaeus monodon. The deduced precursor sequence consists of a signal peptide, B chain, C peptide and an A chain. It exhibits the same structural organization as that of previously identified crustacean insulin-like androgenic gland specific peptides (IAGs). The positions of cysteine residues of the putative A and B chains, which govern the folding of the mature peptide via the formation of disulfide bridges, are highly conserved among the prawn and other crustaceans, while the rest of the amino acids show low sequence similarity. Gene expression analysis of Pm-IAG in several tissues, including the closely juxtaposed sperm duct and muscle, confirmed that it is specifically expressed in the AG. The findings suggest that with an appropriate intervention, sexual differentiation could be manipulated and thus might be instrumental for the establishment of monosex culture in this bimodally growing shrimp.

Details

Title: Isolation and characterization of the complete cDNA sequence encoding a putative insulin-like peptide from the androgenic gland of Penaeus monodon
Authors: Vijay R Mareddy (Author) - University of the Sunshine Coast - Faculty of Science, Health and Education
O Rosen (Author) - Ben-Gurion University of the Negev, Israel
H Thaggard (Author) - Department of Employment, Economic Development and Innovation
R Manor (Author) - Ben-Gurion University of the Negev, Israel
Anna V Kuballa (Author) - University of the Sunshine Coast - Faculty of Science, Health and Education
E D Aflalo (Author) - Ben-Gurion University of the Negev, Israel
A Sagi (Author) - Ben-Gurion University of the Negev, Israel
B Paterson (Author) - Department of Employment, Economic Development and Innovation
Abigail Elizur (Author) - University of the Sunshine Coast - Faculty of Science, Health and Education
Publication details: Aquaculture, Vol.318(3-4), pp.364-370
Publisher: Elsevier BV
Date published: 2011
DOI: 10.1016/j.aquaculture.2011.05.027
ISSN: 0044-8486
Organisation Unit: School of Health - Biomedicine; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy; School of Health and Sport Sciences - Legacy; Centre for Bioinnovation; School of Health and Behavioural Sciences - Legacy
Language: English
Record Identifier: 99449708602621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Fisheries; Marine & Freshwater Biology

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