Isolation and characterization of microsatellite loci in Santalum lanceolatum and Santalum leptocladum (Santalaceae)

B L Jones; M Waycott; H L A Robson; A Calladine; Tony Page

doi:10.3732/ajb.1000213

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Isolation and characterization of microsatellite loci in Santalum lanceolatum and Santalum leptocladum (Santalaceae)

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Isolation and characterization of microsatellite loci in Santalum lanceolatum and Santalum leptocladum (Santalaceae)

B L Jones, M Waycott, H L A Robson, A Calladine and Tony Page

American Journal of Botany, Vol.97(10), pp.97-98

2010

DOI: https://doi.org/10.3732/ajb.1000213

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Abstract

Sandalwood

Santalum

microsatellite

cross-species amplification

Premise of the study: Microsatellite primers were developed for the first time in the native Australian sandalwood species Santalum lanceolatum. Methods and Results: Using an enrichment cloning protocol, five novel polymorphic codominant loci were developed and characterized in S. lanceolatum and S. leptocladum. In addition to these, three existing microsatellite loci from other sandal-wood species were successfully amplified and characterized for S. lanceolatum and S. leptocladum. Among the eight loci, al-lelic diversity ranged from 4 to 29. Conclusions: Primers will be useful for studies of clonality, genetic diversity and spatial genetic structure in wild populations. When coupled with other molecular techniques will help investigate the relationship between S. lanceolatum and S. lepto-cladum, species of commercial and conservation interest. © 2010 Botanical Society of America.

Details

Title: Isolation and characterization of microsatellite loci in Santalum lanceolatum and Santalum leptocladum (Santalaceae)
Authors: B L Jones (Author) - James Cook University
M Waycott (Author) - James Cook University
H L A Robson (Author) - James Cook University
A Calladine (Author) - James Cook University
Tony Page (Author) - James Cook University
Publication details: American Journal of Botany, Vol.97(10), pp.97-98
Publisher: Botanical Society of America, Inc.
Date published: 2010
DOI: 10.3732/ajb.1000213
ISSN: 0002-9122
Organisation Unit: Australian Centre for Pacific Islands Research; Tropical Forests and People Research Centre; University of the Sunshine Coast, Queensland; Forest Industries Research Centre; Forest Research Institute
Language: English
Record Identifier: 99449357402621
Output Type: Journal article

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