Isolation and characterization of a female-specific DNA marker in the giant freshwater prawn Macrobrachium rosenbergii

Tomer Ventura; E D Aflalo; S Weil; K Kashkush; A Sagi

doi:10.1038/hdy.2011.32

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Isolation and characterization of a female-specific DNA marker in the giant freshwater prawn Macrobrachium rosenbergii

Journal article

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Isolation and characterization of a female-specific DNA marker in the giant freshwater prawn Macrobrachium rosenbergii

Tomer Ventura, E D Aflalo, S Weil, K Kashkush and A Sagi

Heredity, Vol.107(5), pp.456-461

2011

DOI: https://doi.org/10.1038/hdy.2011.32

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Abstract

crustacean

prawn

sex DNA marker

sex chromosomes

AFLP

In this study, a female-specific DNA marker in the freshwater prawn Macrobrachium rosenbergii was identified through amplified fragment length polymorphism (AFLP). The AFLPderived sequence-characterized amplified region (SCAR) marker was tested in over 200 individuals, giving reproducible sex identification. Further molecular characterization of the sex-marker's genomic region (B3 kb long) revealed the presence of tandem and inverted repeats. The B3-kb sequence was identified both in male and female prawns, but with subtle differences: a deletion of 3 bp (present in female prawn but absent in male prawn) identified upstream of the SCAR marker sequence and two female-specific single-nucleotide polymorphisms, both indicating that male prawns are homozygous, whereas female prawns are heterozygous in this locus. Fluorescent in situ hybridization showed the B3-kb sequence to be unique: to the best of our knowledge, this is the first report of a unique sex-specific sequence observed in situ in crustaceans. The sex-specific marker identified in M. rosenbergii may have considerable applied merit for crustacean culture in that it will enable the determination of genetic sex at early developmental stages when phenotypic differences are not identifiable.

Details

Title: Isolation and characterization of a female-specific DNA marker in the giant freshwater prawn Macrobrachium rosenbergii
Authors: Tomer Ventura (Author) - Ben-Gurion University of the Negev, Israel
E D Aflalo (Author) - Ben-Gurion University of the Negev, Israel
S Weil (Author) - Ben-Gurion University of the Negev, Israel
K Kashkush (Author) - Ben-Gurion University of the Negev, Israel
A Sagi (Author) - Ben-Gurion University of the Negev, Israel
Publication details: Heredity, Vol.107(5), pp.456-461
Publisher: Nature Publishing Group
Date published: 2011
DOI: 10.1038/hdy.2011.32
ISSN: 0018-067X
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99450018902621
Output Type: Journal article

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