Isolation and characterisation of novel microsatellite and mitochondrial DNA markers for the Eastern Water Dragon (Physignathus lesueurii)

Celine H Frere; P J Prentis; T Ezaz; A Georges

doi:10.1007/s12686-011-9487-3

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Isolation and characterisation of novel microsatellite and mitochondrial DNA markers for the Eastern Water Dragon (Physignathus lesueurii)

Journal article

Peer reviewed

Isolation and characterisation of novel microsatellite and mitochondrial DNA markers for the Eastern Water Dragon (Physignathus lesueurii)

Celine H Frere, P J Prentis, T Ezaz and A Georges

Conservation Genetics Resources, Vol.4(1), pp.113-116

2012

DOI: https://doi.org/10.1007/s12686-011-9487-3

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Abstract

paternity

agamid lizards

agamidae

gene flow

kinship

454 pryosequencing

Habitat fragmentation as a result of urbanisation is a growing problem for native lizard species. The Eastern Water Dragon (Physignathus lesueurii) is a social arboreal agamid lizard, native to Australia. This species represents an ideal model species to investigate the effect of urbanisation because of their prominent abundance in the urban landscape. Here we describe the isolation and characterisation of a novel set of 74 di-, tri-, and tetramicrosatellites from which 18 were selected and optimised into two multiplexes. The 18 microsatellites generated a total 148 alleles across the two populations. The number of alleles per locus varied from 2 to 18 alleles and measures of Ho and He varied from 0.395 to 0.877 and from 0.441 to 0.880, respectively. We also present primers for four novel mitochondrial DNA (mtDNA) markers. The combined length of the four mtDNA marker pairs was 2,528 bp which included 15 nucleotides changes. In comparison to threatened species, which are generally characterised by small population sizes, the Eastern Water Dragon represents an ideal model species to investigate the effect of urbanisation on their behavioural ecology and connectivity patterns among populations.

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Title: Isolation and characterisation of novel microsatellite and mitochondrial DNA markers for the Eastern Water Dragon (Physignathus lesueurii)
Authors: Celine H Frere (Author) - University of Queensland
P J Prentis (Author) - Queensland University of Technology
T Ezaz (Author) - University of Canberra
A Georges (Author) - University of Canberra
Publication details: Conservation Genetics Resources, Vol.4(1), pp.113-116
Publisher: Springer Netherlands
Date published: 2012
DOI: 10.1007/s12686-011-9487-3
ISSN: 1877-7252
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering
Language: English
Record Identifier: 99447757302621
Output Type: Journal article

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Collaboration types: Domestic collaboration
Web Of Science research areas: Biodiversity Conservation; Genetics & Heredity