Isolation and Antimicrobial Susceptibilities of Chlamydial Isolates from Western Barred Bandicoots

S Kumar; A Kutlin; P Roblin; S Kohlhoff; T Bodetti; Peter Timms; M Hammerschlag

doi:10.1128/JCM.01726-06

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Isolation and Antimicrobial Susceptibilities of Chlamydial Isolates from Western Barred Bandicoots

Journal article

Open access

Peer reviewed

Isolation and Antimicrobial Susceptibilities of Chlamydial Isolates from Western Barred Bandicoots

S Kumar, A Kutlin, P Roblin, S Kohlhoff, T Bodetti, Peter Timms and M Hammerschlag

Journal of Clinical Microbiology, Vol.45(2), pp.392-394

2007

DOI: https://doi.org/10.1128/JCM.01726-06

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Abstract

Biological Sciences

Agricultural and Veterinary Sciences

Medical and Health Sciences

chlamydia infection

anti-bacerial agents

marsupialia

chlamydophila pneumoniae

A range of species of Chlamydiales have previously been detected in a variety of Australian marsupials, including koalas and western barred bandicoots. Thirty-seven ocular, urogenital, or nasal swabs were obtained from 21 wild western barred bandicoots. Chlamydia culture and antibiotic susceptibility testing were performed for cycloheximide-treated HEp-2 cells in 96-well microtiter plates. Chlamydia spp. were isolated from 11 specimens from 9 (42.8%) bandicoots. All isolates were identified as Chlamydiales by conventional PCR with 16S and 23S rRNA gene primers specific to Chlamydiales and were confirmed to be Chlamydia pneumoniae by a C. pneumoniae-specific ompA-based real-time PCR assay and 16S rRNA and 23S rRNA gene signature sequence analyses. The MICs of azithromycin, doxycycline, ciprofloxacin, and enrofloxacin for 10 C. pneumoniae isolates from these bandicoots ranged from 0.015 to 1 μg/ml, 0.25 to 1 μg/ml, 0.25 to 2 μg/ml, and 0.25 to 0.5 μg/ml, respectively. The MICs at which 90% of isolates were inhibited and the minimal bactericidal concentrations were within the ranges reported previously for human isolates of C. pneumoniae.

Details

Title: Isolation and Antimicrobial Susceptibilities of Chlamydial Isolates from Western Barred Bandicoots
Authors: S Kumar (Author) - State University of New York, United States
A Kutlin (Author) - State University of New York, United States
P Roblin (Author) - State University of New York, United States
S Kohlhoff (Author) - State University of New York, United States
T Bodetti (Author) - Queensland University of Technology
Peter Timms (Author) - Queensland University of Technology
M Hammerschlag (Author) - State University of New York, United States
Publication details: Journal of Clinical Microbiology, Vol.45(2), pp.392-394
Publisher: American Society for Microbiology
Date published: 2007
DOI: 10.1128/JCM.01726-06
ISSN: 0095-1137
Copyright note: Copyright © 2007 American Society for Microbiology. The author's accepted version is reproduced here in accordance with the publisher's copyright policy. The definitive version is available at http://aem.asm.org/
Organisation Unit: University of the Sunshine Coast, Queensland; Centre for Bioinnovation
Language: English
Record Identifier: 99449116802621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Microbiology

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