Isoform specific changes in PPARα and β in colon and breast cancer with differentiation

Cho S Aung; Helen Faddy; Erin J Lister; Gregory R Monteith; Sarah J Roberts-Thomson

doi:10.1016/j.bbrc.2005.12.061

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Isoform specific changes in PPARα and β in colon and breast cancer with differentiation

Journal article

Peer reviewed

Isoform specific changes in PPARα and β in colon and breast cancer with differentiation

Cho S Aung, Helen Faddy, Erin J Lister, Gregory R Monteith and Sarah J Roberts-Thomson

Biochemical and Biophysical Research Communications, Vol.340(2), pp.656-660

2006

DOI: https://doi.org/10.1016/j.bbrc.2005.12.061

PMID: 16378595

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https://doi.org/10.1016/j.bbrc.2005.12.061View

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Abstract

Human

Post-confluence

Sodium butyrate

PPAR

RT-PCR

Cell lines

Breast

Alkaline phosphatase intestinal

Colon

Differentiation

To investigate the role of peroxisome proliferator-activated receptors (PPARs) α and β in the differentiation of colon cancer cells, we differentiated HT-29 cells using sodium butyrate (NaB) and culturing post-confluence and assessed differentiation using the marker intestinal alkaline phosphatase. While PPARα levels only changed with culturing post confluence, PPARβ levels increased independent of the method of differentiation. To explore further the differences induced by NaB, we assessed changes in both PPAR isoforms in MCF-7 breast cancer cells cultured in the presence of NaB over 48 h. Again a very different expression pattern was observed with PPARα increasing after 4 h and remaining elevated, while PPARβ increased transiently. Our studies suggest that the expression of PPARs is dependent upon both the method of differentiation and on time. Moreover, these studies show that changes in PPARα levels are not required for the differentiation of colon cancer cell lines, whereas changes in PPARβ are more closely associated with differentiation.

Details

Title: Isoform specific changes in PPARα and β in colon and breast cancer with differentiation
Authors: Cho S Aung (Author) - University of Queensland
Helen Faddy (Author) - University of Queensland
Erin J Lister (Author) - University of Queensland
Gregory R Monteith (Author) - University of Queensland
Sarah J Roberts-Thomson (Author) - University of Queensland
Publication details: Biochemical and Biophysical Research Communications, Vol.340(2), pp.656-660
Publisher: Elsevier Inc.
DOI: 10.1016/j.bbrc.2005.12.061
ISSN: 1090-2104
PMID: 16378595
Organisation Unit: School of Health and Behavioural Sciences - Legacy; School of Health and Sport Sciences - Legacy; University of the Sunshine Coast, Queensland; School of Health - Biomedicine
Language: English
Record Identifier: 99477806402621
Output Type: Journal article

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