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Ionotropic receptors in the turnip moth Agrotis segetum respond to repellent medium-chain fatty acids6.57 MB
Published VersionCC BY V4.0, Open Access
Journal article
Ionotropic receptors in the turnip moth Agrotis segetum respond to repellent medium-chain fatty acids
BMC Biology, Vol.20, pp.1-19
2022
PMID: 35130883
Abstract
Background:
In insects, airborne chemical signals are mainly detected by two receptor families, odorant receptors (ORs) and ionotropic receptors (IRs). Functions of ORs have been intensively investigated in Diptera and Lepidoptera, while the functions and evolution of the more ancient IR family remain largely unexplored beyond Diptera.
Results:
Here, we identified a repertoire of 26 IRs from transcriptomes of female and male antennae, and ovipositors in the moth Agrotis segetum. We observed that a large clade formed by IR75p and IR75q expansions is closely related to the acid-sensing IRs identified in Diptera. We functionally assayed each of the five AsegIRs from this clade using Xenopus oocytes and found that two receptors responded to the tested ligands. AsegIR75p.1 responded to several compounds but hexanoic acid was revealed to be the primary ligand, and AsegIR75q.1 responded primarily to octanoic acid, and less so to nonanoic acid. It has been reported that the C6-C10 medium-chain fatty acids repel various insects including many drosophilids and mosquitos. We show that the C6-C10 medium-chain fatty acids elicited antennal responses of both sexes of A. segetum, while only octanoic acid had repellent effect to the moths in a behavioral assay. In addition, using fluorescence in situ hybridization, we demonstrated that the five IRs and their co-receptor AsegIR8a are not located in coeloconic sensilla as found in Drosophila, but in basiconic or trichoid sensilla.
Conclusions:
Our results significantly expand the current knowledge of the insect IR family. Based on the functional data in combination with phylogenetic analysis, we propose that subfunctionalization after gene duplication plays an important role in the evolution of ligand specificities of the acid-sensing IRs in Lepidoptera.
Details
- Title
- Ionotropic receptors in the turnip moth Agrotis segetum respond to repellent medium-chain fatty acids
- Authors
- Xiao-Qing Hou (Author) - Lund UniversityDan-Dan Zhang (Author) - Lund UniversityDaniel Powell (Author) - Lund UniversityHong-Lei Wang (Author) - Lund UniversityMartin N Andersson (Author) - Lund UniversityChrister Löfstedt (Author) - Lund University
- Publication details
- BMC Biology, Vol.20, pp.1-19
- Publisher
- BioMed Central Ltd.
- Date published
- 2022
- DOI
- 10.1186/s12915-022-01235-0
- ISSN
- 1741-7007
- PMID
- 35130883
- Copyright note
- This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
- Organisation Unit
- University of the Sunshine Coast, Queensland; Office of Research; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99638478802621
- Output Type
- Journal article
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