Files and links (1)
Investigation of Best Practices for Venom Toxin Purification in Jellyfish towards Functional Characterisation2.21 MB
Published VersionCC BY V4.0, Open Access
Journal article
Investigation of Best Practices for Venom Toxin Purification in Jellyfish towards Functional Characterisation
Toxins, Vol.15(3), pp.1-27
2023
PMID: 36977061
Abstract
The relative lack of marine venom pharmaceuticals can be anecdotally attributed to difficulties in working with venomous marine animals, including how to maintain venom bioactivity during extraction and purification. The primary aim of this systematic literature review was to examine the key factors for consideration when extracting and purifying jellyfish venom toxins to maximise their effectiveness in bioassays towards the characterisation of a single toxin.An up to date database of 119 peer reviewed research articles was established for all purified and semi purified venoms across all jellyfish, including their level of purification, LD50, and the types of experimental toxicity bioassay used (e.g., whole animal and cell lines). We report that, of the toxins successfully purified across all jellyfish, the class Cubozoa (i.e., Chironex fleckeri and Carybdea rastoni) was most highly represented, followed by Scyphozoa and Hydrozoa. We outline the best practices for maintaining jellyfish venom bioactivity, including strict thermal management, using the “autolysis” extraction method and two-step liquid chromatography purification involving size exclusion chromatography. To date, the box jellyfish C. fleckeri has been the most effective jellyfish venom model with the most referenced extraction methods and the most isolated toxins, including CfTXA/B. In summary, this review can be used as a resource for the efficient extraction, purification, and identification of jellyfish venom toxins.
Details
- Title
- Investigation of Best Practices for Venom Toxin Purification in Jellyfish towards Functional Characterisation
- Authors
- Blake Lausen (Author) - University of the Sunshine Coast, Queensland, School of Health - BiomedicineAnahita Ahang (Author) - University of the Sunshine Coast, Queensland, Centre for BioinnovationScott Cummins (Author) - University of the Sunshine Coast, Queensland, Centre for BioinnovationTianfang Wang (Corresponding Author) - University of the Sunshine Coast, Queensland, Centre for Bioinnovation
- Publication details
- Toxins, Vol.15(3), pp.1-27
- Publisher
- MDPI AG
- Date published
- 2023
- DOI
- 10.3390/toxins15030170
- ISSN
- 2072-6651
- PMID
- 36977061
- Copyright note
- © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/ 4.0/).
- Organisation Unit
- School of Health - Biomedicine; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99714998602621
- Output Type
- Journal article
Metrics
37 File views/ downloads
63 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Web Of Science research areas
- Food Science & Technology
- Toxicology