Investigating the theragnostic potential of 131I-caerin peptide in thyroid cancer

Ying-Ying Zhou; Pei-Pei Zhang; Ruo-Ting Lin; Tong-Sheng Chen; Xiong-Ying Liu; Wen-Juan Liu; Yong-Nan Liang; Shu Chen; Xuan Pan; Guoying Ni; Tianfang Wang; Xiao Song Liu; Jian-Wei Yuan

doi:10.1967/s002449912003

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Investigating the theragnostic potential of 131I-caerin peptide in thyroid cancer

Journal article

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Investigating the theragnostic potential of 131I-caerin peptide in thyroid cancer

Ying-Ying Zhou, Pei-Pei Zhang, Ruo-Ting Lin, Tong-Sheng Chen, Xiong-Ying Liu, Wen-Juan Liu, Yong-Nan Liang, Shu Chen, Xuan Pan, Guoying Ni, …

Hellenic Journal of Nuclear Medicine, Vol.23(1), pp.27-33

2020

DOI: https://doi.org/10.1967/s002449912003

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Abstract

Oncology and Carcinogenesis

caerin peptide

caerin

thyroid cancer

Objective: Caerin is a new peptide with tumour toxicity and its uptake by tumour cells is independent of the sodium iodide symporter (NIS). Thyroid cancer is the most common cancers of endocrine malignancy. Radioiodine (131I)-refractory thyroid cancer is the most lethal subtype of the thyroid cancers and remains a clinical challenge. In the current study, we investigated the 131I radiolabeling efficiency of Caerin and the effects of Caerin, 131I-Caerin and free 131I on differentiated and undifferentiated human thyroid cancer cell lines (B-CPAP and CAL-62) in vitro. Materials and methods: Cell Counting Kit-8 was used to assess the cytotoxic effect of Caerin, 131I-Caerin and free 131I on B-CPAP and CAL-62 cells. Laser scanning confocal microscope was exploited to evaluate the uptake and internalization of Caerin by thyroid cancer cells. The Chloramine-T method was used to label the peptide with 131I. And the stability and water partition coefficient (Log P) of 131I-Caerin were studied. Results: Our results demonstrated that Caerin and 131I-Caerin could be accumulated by B-CPAP and CAL-62 cells, resulting in killing of the thyroid cancer cells in vitro. The efficacy of 131I-Caerin is much higher than 131I, especially to undifferentiated CAL-62 cells. The results prove the feasibility of radioiodination of the 131I-Caerin via the Chloramine-T method. Moreover, the result indicate the hydrophobic 131I-Caerin was stable in 72 hours. Conclusion: Iodine-131-Caerin can inhibit the cell viability of thyroid cancer and hold certain promise as a theragnostic tool for human thyroid cancers.

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Title: Investigating the theragnostic potential of 131I-caerin peptide in thyroid cancer
Authors: Ying-Ying Zhou (Author) - Duangdong Pharmaceutical University, China
Pei-Pei Zhang (Author) - First People's Hospital, China
Ruo-Ting Lin (Author) - Duangdong Pharmaceutical University, China
Tong-Sheng Chen (Author) - Duangdong Pharmaceutical University, China
Xiong-Ying Liu (Author) - Duangdong Pharmaceutical University, China
Wen-Juan Liu (Author) - Duangdong Pharmaceutical University, China
Yong-Nan Liang (Author) - Duangdong Pharmaceutical University, China
Shu Chen (Author) - First People's Hospital, China
Xuan Pan (Author) - Duangdong Pharmaceutical University, China
Guoying Ni (Author) - University of the Sunshine Coast - School of Science and Engineering
Tianfang Wang (Author) - University of the Sunshine Coast - School of Science and Engineering
Xiao Song Liu (Author) - University of the Sunshine Coast - School of Science and Engineering
Jian-Wei Yuan (Author) - Duangdong Pharmaceutical University, China
Publication details: Hellenic Journal of Nuclear Medicine, Vol.23(1), pp.27-33
Publisher: Hellenic Society of Nuclear Medicine
Date published: 2020
DOI: 10.1967/s002449912003
ISSN: 1108-1430; 1790-5427
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99450947602621
Output Type: Journal article

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