Intracellular sucrose communicates metabolic demand to sucrose transporters in developing pea cotyledons

Yuchan Zhou; Katie Chan; Trevor L Wang; C L Hedley; C E Offler; J W Patrick

doi:10.1093/jxb/ern254

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Intracellular sucrose communicates metabolic demand to sucrose transporters in developing pea cotyledons

Journal article

Peer reviewed

Intracellular sucrose communicates metabolic demand to sucrose transporters in developing pea cotyledons

Yuchan Zhou, Katie Chan, Trevor L Wang, C L Hedley, C E Offler and J W Patrick

Journal of Experimental Botany, Vol.60(1), pp.71-85

2009

DOI: https://doi.org/10.1093/jxb/ern254

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Abstract

cotyledon

hexose

pea

rugosus loci

seed development

sucrose

sucrose transporter

Mechanistic inter-relationships in sinks between sucrose compartmentation/metabolism and phloem unloading/translocation are poorly understood. Developing grain legume seeds provide tractable experimental systems to explore this question. Metabolic demand by cotyledons is communicated to phloem unloading and ultimately import by sucrose withdrawal from the seed apoplasmic space via a turgor-homeostat mechanism. What is unknown is how metabolic demand is communicated to cotyledon sucrose transporters responsible for withdrawing sucrose from the apoplasmic space. This question was explored here using a pea rugosus mutant (rrRbRb) compromised in starch biosynthesis compared with its wild-type counterpart (RRRbRb). Sucrose influx into cotyledons was found to account for 90% of developmental variations in their absolute growth and hence starch biosynthetic rates. Furthermore, rr and RR cotyledons shared identical response surfaces, indicating that control of transporter activity was likely to be similar for both lines. In this context, sucrose influx was correlated positively with expression of a sucrose/H+ symporter (PsSUT1) and negatively with two sucrose facilitators (PsSUF1 and PsSUF4). Sucrose influx exhibited a negative curvilinear relationship with cotyledon concentrations of sucrose and hexoses. In contrast, the impact of intracellular sugars on transporter expression was transporter dependent, with expression of PsSUT1 inhibited, PsSUF1 unaffected, and PsSUF4 enhanced by sugars. Sugar supply to, and sugar concentrations of, RR cotyledons were manipulated using in vitro pod and cotyledon culture. Collectively the results obtained showed that intracellular sucrose was the physiologically active sugar signal that communicated metabolic demand to sucrose influx and this transport function was primarily determined by PsSUT1 regulated at the transcriptional level. © 2008 The Author(s).

Details

Title: Intracellular sucrose communicates metabolic demand to sucrose transporters in developing pea cotyledons
Authors: Yuchan Zhou (Author) - University of Newcastle
Katie Chan (Author) - University of Newcastle
Trevor L Wang (Author) - John Innes Centre, United Kingdom
C L Hedley (Author) - John Innes Centre, United Kingdom
C E Offler (Author) - University of Newcastle
J W Patrick (Author) - University of Newcastle
Publication details: Journal of Experimental Botany, Vol.60(1), pp.71-85
Publisher: Oxford University Press
Date published: 2009
DOI: 10.1093/jxb/ern254
ISSN: 0022-0957
Organisation Unit: Australian Centre for Pacific Islands Research; University of the Sunshine Coast, Queensland
Language: English
Record Identifier: 99449347102621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Plant Sciences

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