Intestinal Transcriptome Analysis Highlights Key Differentially Expressed Genes Involved in Nutrient Metabolism and Digestion in Yellowtail Kingfish (Seriola lalandi) Fed Terrestrial Animal and Plant Proteins

Chinh Thi My Dam; Tomer Ventura; Mark Booth; Igor Pirozzi; Michael Salini; Richard Smullen; Abigail Elizur

doi:10.3390/genes11060621

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Intestinal Transcriptome Analysis Highlights Key Differentially Expressed Genes Involved in Nutrient Metabolism and Digestion in Yellowtail Kingfish (Seriola lalandi) Fed Terrestrial Animal and Plant Proteins

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Intestinal Transcriptome Analysis Highlights Key Differentially Expressed Genes Involved in Nutrient Metabolism and Digestion in Yellowtail Kingfish (Seriola lalandi) Fed Terrestrial Animal and Plant Proteins

Chinh Thi My Dam, Tomer Ventura, Mark Booth, Igor Pirozzi, Michael Salini, Richard Smullen and Abigail Elizur

Genes, Vol.11(6), pp.1-17

2020

DOI: https://doi.org/10.3390/genes11060621

PMCID: PMC7349653

PMID: 32517020

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Abstract

yellowtail kingfish

RNA-sequencing

transcriptomes

alternative protein sources

distal intestine

digestion

This study investigated the effects of dietary terrestrial animal and plant proteins on the intestinal transcriptomes of yellowtail kingfish (YTK), Seriola lalandi, an ecologically and economically important marine species in Australia. Five diets containing fish meal (FM), poultry by-product meal (PBM), blood meal (BLM), faba bean meal (FBM) and corn gluten meal (CGM) were formulated and fed over a period of 4 weeks. The Illumina RNA-sequencing (RNA-Seq) results identified a suite of differentially expressed genes involved in nutrient metabolism and protein digestion pathways, reinforced by quantitative polymerase chain reaction (qPCR) results. These findings provide molecular support to the notion that PBM and FBM are useful raw materials in commercial diets for YTK. Using the same evidence, we have demonstrated that BLM and CGM may be less useful and their incorporation into commercial aquafeeds for this species should be done cautiously. The differentially expressed genes showed a subtle difference and high correlation with apparent nutrient digestibility of raw materials. Further, our results indicate that transcriptome profiling provides a useful tool to evaluate alternative protein sources for use in aquaculture feeds.

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Title: Intestinal Transcriptome Analysis Highlights Key Differentially Expressed Genes Involved in Nutrient Metabolism and Digestion in Yellowtail Kingfish (Seriola lalandi) Fed Terrestrial Animal and Plant Proteins
Authors: Chinh Thi My Dam (Author) - University of the Sunshine Coast, Queensland, School of Science and Engineering - Legacy
Tomer Ventura (Author) - University of the Sunshine Coast, Queensland, School of Science and Engineering - Legacy
Mark Booth (Author) - New South Wales Department of Primary Industries
Igor Pirozzi (Author) - James Cook University
Michael Salini (Author) - Ridley Aquafeed Pty Ltd
Richard Smullen (Author) - Ridley Aquafeed Pty Ltd
Abigail Elizur (Author) - University of the Sunshine Coast, Queensland, GeneCology Research Centre - Legacy
Publication details: Genes, Vol.11(6), pp.1-17
Publisher: MDPI AG
Date published: 2020
DOI: 10.3390/genes11060621
PMID: 32517020; PMC7349653
Copyright note: Copyright © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99465408502621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Genetics & Heredity