Interleukin-13 Induces Dramatically Different Transcriptional Programs in Three Human Airway Cell Types

J H Lee; N Kaminski; G Dolganov; G Grunig; L Koth; Colin Solomon; D J Erle; D Sheppard

doi:10.1165/ajrcmb.25.4.4522

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Interleukin-13 Induces Dramatically Different Transcriptional Programs in Three Human Airway Cell Types

Journal article

Peer reviewed

Interleukin-13 Induces Dramatically Different Transcriptional Programs in Three Human Airway Cell Types

J H Lee, N Kaminski, G Dolganov, G Grunig, L Koth, Colin Solomon, D J Erle and D Sheppard

American Journal of Respiratory Cell and Molecular Biology, Vol.25(4), pp.474-485

2001

DOI: https://doi.org/10.1165/ajrcmb.25.4.4522

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http://ajrcmb.atsjournals.org/content/25/4/474.full.pdf+html?sid=cce3ce55-36f5-4171-a7c6-774b1d871641View

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Abstract

Cardiovascular Medicine and Haematology

Interleukin (IL)-13, a cytokine released by T lymphocytes during immediate hypersensitivity responses, is a central mediator of asthma. Because IL-13 induces phenotypic features of asthma in mice deficient in T and B lymphocytes, it is likely that this cytokine contributes to the development of asthma by acting directly on resident airway cells. To analyze the global effects of IL-13 on gene expression in airway cells that could contribute to the phenotypic features of asthma, we used Genechip HuGene FL arrays (Affymetrix, Santa Clara, CA) that contain probes for approximately 6,500 human genes. Despite activating a common signaling pathway, IL-13 induced dramatically different patterns of gene expression in primary cultures of airway epithelial cells, airway smooth muscle cells, and lung fibroblasts, with little overlap among cell types. The most prominent effects of IL-13 were on airway smooth muscle, but several genes induced in airway epithelial cells and fibroblasts are also candidates that may contribute to phenotypic features of asthma. These results suggest that the in vivo response to IL-13 in the airways likely results from a combination of distinct effects on each of several resident airway cell types.

Details

Title: Interleukin-13 Induces Dramatically Different Transcriptional Programs in Three Human Airway Cell Types
Authors: J H Lee (Author)
N Kaminski (Author)
G Dolganov (Author)
G Grunig (Author)
L Koth (Author)
Colin Solomon (Author) - University of California, United States
D J Erle (Author)
D Sheppard (Author)
Publication details: American Journal of Respiratory Cell and Molecular Biology, Vol.25(4), pp.474-485
Publisher: American Thoracic Society
Date published: 2001
DOI: 10.1165/ajrcmb.25.4.4522
ISSN: 1044-1549
Organisation Unit: Centre for Human Factors and Systems Science; University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; School of Health - Sports & Exercise Science; School of Health and Behavioural Sciences - Legacy
Language: English
Record Identifier: 99450247402621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Biochemistry & Molecular Biology; Cell Biology; Respiratory System

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