Journal article
Insights Into Sexual Maturation and Reproduction in the Norway Lobster (Nephrops norvegicus) via in Silico Prediction and Characterization of Neuropeptides and G Protein-coupled Receptors
Frontiers in Endocrinology, Vol.9, 430
2018
PMCID: PMC6073857
PMID: 30100897
Abstract
Multiple biological processes across development and reproduction are modulated by neuropeptides that are predominantly produced and secreted from an animal's central nervous system. In the past few years, advancement of next-generation sequencing technologies has enabled large-scale prediction of putative neuropeptide genes in multiple non-model species, including commercially important decapod crustaceans. In contrast, knowledge of the G protein-coupled receptors (GPCRs), through which neuropeptides act on target cells, is still very limited. In the current study, we have used in silico transcriptome analysis to elucidate genes encoding neuropeptides and GPCRs in the Norway lobster (Nephrops norvegicus), which is one of the most valuable crustaceans in Europe. Fifty-seven neuropeptide precursor-encoding transcripts were detected, including phoenixin, a vertebrate neurohormone that has not been detected in any invertebrate species prior to this study. Neuropeptide gene expression analysis of immature and mature female N. norvegicus, revealed that some reproduction-related neuropeptides are almost exclusively expressed in immature females. In addition, a total of 223 GPCR-encoding transcripts were identified, of which 116 encode GPCR-A (Rhodopsin), 44 encode GPCR-B (Secretin) and 63 encode other GPCRs. Our findings increase the molecular toolbox of neural signaling components in N. norvegicus, allowing for further advances in the fisheries/larvae culture of this species.
Details
- Title
- Insights Into Sexual Maturation and Reproduction in the Norway Lobster (Nephrops norvegicus) via in Silico Prediction and Characterization of Neuropeptides and G Protein-coupled Receptors
- Authors
- Tuan Viet Nguyen (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringGuiomar E Rotllant (Author) - Passeig Maritim de la Barceloneta, SpainScott F Cummins (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringAbigail Elizur (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringTomer Ventura (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
- Publication details
- Frontiers in Endocrinology, Vol.9, 430; 16
- Publisher
- Frontiers Research Foundation
- Date published
- 2018
- DOI
- 10.3389/fendo.2018.00430
- ISSN
- 1664-5292
- PMID
- 30100897; PMC6073857
- Copyright note
- Copyright © 2018 Nguyen, Rotllant, Cummins, Elizur and Ventura. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
- Organisation Unit
- School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy; USC International - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99451497302621
- Output Type
- Journal article
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