Journal article
Influence of cuff-occlusion duration on contrast-enhanced ultrasound assessments of calf muscle microvascular blood flow responsiveness in older adults
Experimental Physiology, Vol.105(12), pp.2238-2245
2020
Abstract
Contrast‐enhanced ultrasound (CEUS) is increasingly used in assessments of skeletal muscle microvascular blood flow responsiveness. In response to limb cuff‐occlusion, some studies have reported significant impairments in CEUS measurements of microvascular blood flow in older adults with cardiovascular or metabolic disease, whereas others have failed to detect significant between‐group differences, which has brought the reliability of the technique into question. In the absence of a standardized CEUS protocol, there is variance in the duration of cuff‐occlusion used, which is likely to influence post‐occlusion measurements of muscle microvascular blood flow. We aimed to determine the effect of cuff‐occlusion duration by comparing the magnitude and reliability of CEUS measurements of calf muscle microvascular blood flow responsiveness in older adults (n = 15, 67 ± 11 years) following 1, 3 and 5 min occlusion periods. Microvascular blood flow (= microvascular volume × microvascular velocity) within the calf muscle was measured using real‐time destruction–replenishment CEUS. Measurements were made following thigh cuff‐occlusion (200 mmHg) periods of 1, 3 and 5 min in a random order. Microvascular blood flow was higher following 3 min (3.71 ± 1.46 aU s−1) and 5 min (3.47 ± 1.48 aU s−1) compared with 1 min (2.42 ± 1.27 aU s−1, P = 0.002), which corresponded with higher microvascular volumes after 3 and 5 min compared with 1 min. Reliability was good following 5 min (intraclass correlation coefficient (ICC) 0.49) compared with poor following 1 min (ICC 0.34) and 3 min (ICC 0.35). This study demonstrates that the magnitude and reliability of calf muscle microvascular responsiveness is enhanced using a 5 min cuff‐occlusion protocol compared with 1 min in older adults.
Details
- Title
- Influence of cuff-occlusion duration on contrast-enhanced ultrasound assessments of calf muscle microvascular blood flow responsiveness in older adults
- Authors
- Grace Young (Author) - University of the Sunshine Coast, Queensland, School of Health and Sport Sciences - LegacyDigby Krastins (Author) - University of the Sunshine Coast, Queensland, School of Health and Sport Sciences - LegacyDavid Chang (Author) - Sunshine Coast Hospital and Health ServiceJeng Lam (Author) - Sunshine Coast Hospital and Health ServiceJing Quah (Author) - Sunshine Coast University HospitalTony Stanton (Author) - University of the Sunshine CoastFraser Russell (Author) - University of the Sunshine Coast, Queensland, GeneCology Research Centre - LegacyKim Greaves (Author) - University of the Sunshine CoastYuri Kriel (Author) - University of the Sunshine Coast, Queensland, School of Health and Sport Sciences - LegacyChristopher Askew (Corresponding Author) - University of the Sunshine Coast, Queensland, School of Health and Sport Sciences - Legacy
- Publication details
- Experimental Physiology, Vol.105(12), pp.2238-2245
- Publisher
- Wiley-Blackwell Publishing Ltd.
- Date published
- 2020
- DOI
- 10.1113/EP089065
- ISSN
- 1469-445X
- Organisation Unit
- University of the Sunshine Coast, Queensland; Centre for Bioinnovation; Faculty of Science, Health, Education and Engineering; School of Health and Behavioural Sciences - Legacy; School of Health and Sport Sciences - Legacy; School of Nursing, Midwifery and Paramedicine - Legacy; School of Health - Sports & Exercise Science; Centre for Human Factors and Sociotechnical Systems; School of Health - Biomedicine; Cancer Research Cluster
- Language
- English
- Record Identifier
- 99482296902621
- Output Type
- Journal article
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