Journal article
Inflammation and Lung Injury in an Ovine Model of Extracorporeal Membrane Oxygenation Support
American Journal of Physiology: Lung Cellular and Molecular Physiology, Vol.311(6), pp.L1202-L1212
2016
Abstract
Extracorporeal membrane oxygenation (ECMO) is a life-saving treatment for patients with severe, refractory, cardiorespiratory failure. Exposure to the ECMO circuit is thought to trigger/exacerbate inflammation. Determining whether inflammation is due to the patients underlying pathologies or the ECMO circuit is difficult. To discern how different insults contribute to the inflammatory response we developed an ovine model of lung injury and ECMO to investigate the impact of smoke-induced lung injury and ECMO in isolation and cumulatively on pulmonary and circulating inflammatory cells, cytokines and tissue remodeling. Sheep receiving either smoke-induced acute lung injury (S-ALI) or sham injury were placed on veno-venous (VV) ECMO lasting either 2 or 24hrs, with controls receiving conventional ventilation only. Lung tissue, bronchoalveolar fluid and plasma were analyzed by RT-PCR, immunohistochemical staining and zymography to assess inflammatory cells, cytokines and matrix metalloproteinases. Pulmonary compliance decreased in sheep with S-ALI placed on ECMO with increased numbers of infiltrating neutrophils, monocytes and alveolar macrophages compared to controls. Infiltration of neutrophils was also observed with S-ALI alone. RT-PCR studies showed higher expression of MMP2 and MMP9 in S-ALI plus ECMO while IL-6 was elevated at 2hrs. Zymography revealed higher levels of MMP2. Circulating plasma levels of IL-6 were elevated 1-2hrs after commencement of ECMO alone. This data shows that the inflammatory response is enhanced when a host with pre-existing pulmonary injury is placed on ECMO, with increased infiltration of neutrophils and macrophages, the release of inflammatory cytokines and up regulation of matrix metalloproteinases.
Details
- Title
- Inflammation and Lung Injury in an Ovine Model of Extracorporeal Membrane Oxygenation Support
- Authors
- Margaret Passmore (Author) - University of QueenslandYoke Lin Fung (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringGabriela Simonova (Author) - Australian Red Cross Blood ServiceSamuel R Foley (Author) - University of QueenslandKimble R Dunster (Author) - Queensland University of TechnologySara D Diab (Author) - University of QueenslandJohn-Paul Tung (Author) - Australian Red Cross Blood ServiceRobyn M Minchinton (Author)Charles I McDonald (Author) - University of QueenslandChris M Anstey (Author) - University of QueenslandKiran Shekar (Author) - University of QueenslandJohn F Fraser (Author) - University of Queensland
- Publication details
- American Journal of Physiology: Lung Cellular and Molecular Physiology, Vol.311(6), pp.L1202-L1212
- Publisher
- American Physiological Society
- Date published
- 2016
- DOI
- 10.1152/ajplung.00296.2016
- ISSN
- 1040-0605
- Copyright note
- Copyright © 2016 the American Physiological Society
- Organisation Unit
- School of Health - Biomedicine; University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; School of Health and Behavioural Sciences - Legacy
- Language
- English
- Record Identifier
- 99450462702621
- Output Type
- Journal article
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- Domestic collaboration
- Web Of Science research areas
- Physiology
- Respiratory System
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