Induced Spawning of F1 Wreckfish (Hapuku) Polyprion oxygeneios Using a Synthetic Agonist of Gonadotropin-Releasing Hormone

Matthew J Wylie; Alvin N Setiawan; Glen W Irvine; Abigail Elizur; Yonathan Zohar; Jane E Symonds; P Mark Lokman

doi:10.3390/fishes4030041

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Induced Spawning of F1 Wreckfish (Hapuku) Polyprion oxygeneios Using a Synthetic Agonist of Gonadotropin-Releasing Hormone

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Induced Spawning of F1 Wreckfish (Hapuku) Polyprion oxygeneios Using a Synthetic Agonist of Gonadotropin-Releasing Hormone

Matthew J Wylie, Alvin N Setiawan, Glen W Irvine, Abigail Elizur, Yonathan Zohar, Jane E Symonds and P Mark Lokman

Fishes, Vol.4(3), 41

2019

DOI: https://doi.org/10.3390/fishes4030041

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Abstract

Polyprion

spawning induction

oocyte maturation

aquaculture

ovulation

GnRHa

gonadotropin

wreckfish

broodstock

hāpuku

Wild-caught hāpuku (Polyprion oxygeneios) spawn readily in captivity, but although first filial (F1) hāpuku complete vitellogenesis, females fail to undergo oocyte maturation and spawn or produce poor quality eggs. This study investigated whether administration of a synthetic agonist of gonadotropin-releasing hormone (GnRHa) could improve F1 hāpuku spawning and complete the life-cycle in captivity. Spawning trials were conducted over 2 years in 2013 and 2014, when F1 were aged five and six years. In 2013, females previously conditioned under a variable or constant temperature regime were implanted with GnRHa (100 μg/kg−1) or blank implants constructed of powdered cellulose and cholesterol. Spawning was erratic and egg quality very poor in all tanks. No F2 offspring were produced by communal spawning. In contrast, viable F2 larvae were produced by strip-spawning and in vitro fertilization after a series of GnRHa injections. In 2014, two additional trials were conducted: females received ethylene-vinyl acetate copolymer (EVAc) matrix implants containing GnRHa (100 μg/kg−1) or blank implants and in the second trial, two GnRHa doses (100 μg/kg−1 and 50 μg/kg−1) were tested. Eggs were first detected in all tanks 12–17 days post-implantation when females received 100 µg/kg−1 GnRHa implants, but not in the lower dose or control tanks. In summary, this study achieved induction of female spawning with GnRHa implants (target dose 100 μg/kg−1) and the successful production of F2 hāpuku in captivity by strip-spawning.

Details

Title: Induced Spawning of F1 Wreckfish (Hapuku) Polyprion oxygeneios Using a Synthetic Agonist of Gonadotropin-Releasing Hormone
Authors: Matthew J Wylie (Author) - University of Otago, New Zealand
Alvin N Setiawan (Author) - National Institute of Water and Atmospheric Research, New Zealand
Glen W Irvine (Author) - National Institute of Water and Atmospheric Research, New Zealand
Abigail Elizur (Author) - University of the Sunshine Coast
Yonathan Zohar (Author) - University of Maryland, United States
Jane E Symonds (Author) - National Institute of Water and Atmospheric Research, New Zealand
P Mark Lokman (Author) - University of Otago, New Zealand
Publication details: Fishes, Vol.4(3), 41; 16
Publisher: MDPI AG
Date published: 2019
DOI: 10.3390/fishes4030041
ISSN: 2410-3888
Copyright note: Copyright © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Organisation Unit: University of the Sunshine Coast, Queensland; Centre for Bioinnovation
Language: English
Record Identifier: 99450727102621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Fisheries; Marine & Freshwater Biology

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