Increased sensitivity to tryptophan bioavailability in a positive adaptation by the human strains of Chlamydia pneumoniae

Anu Chacko; Christopher J Barker; Kenneth W Beagley; Mark P Hodson; Manuel R Plan; Peter Timms; Wilhelmina M Huston

doi:10.1111/mmi.12701

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Increased sensitivity to tryptophan bioavailability in a positive adaptation by the human strains of Chlamydia pneumoniae

Journal article

Open access

Peer reviewed

Increased sensitivity to tryptophan bioavailability in a positive adaptation by the human strains of Chlamydia pneumoniae

Anu Chacko, Christopher J Barker, Kenneth W Beagley, Mark P Hodson, Manuel R Plan, Peter Timms and Wilhelmina M Huston

Molecular Microbiology, Vol.93(4), pp.797-813

2014

DOI: https://doi.org/10.1111/mmi.12701

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Abstract

Chlamydia pneumoniae

One of the most significant activities induced by interferon-gamma against intracellular pathogens is the induction of IDO (indoleamine 2,3-dioxygenase) expression which subsequently results in the depletion of tryptophan. We tested the hypothesis that human strains of Chlamydia pneumoniae are more sensitive to tryptophan limitation than animal C. pneumoniae strains. The human strains were significantly more sensitive to IFN-γ than the animal strains in a lung epithelia cell model (BEAS-2B), with exposure to 1 U ml-1 IFN-γ resulting in complete loss of infectious yield of human strains, compared to the animal strains where reductions in infectious progeny were around 3.5-4.0 log. Strikingly, the IFN-γ induced loss of ability to form infectious progeny production was completely rescued by removal of the IFN-γ and addition of exogenous tryptophan for the human strains, but not the animal strains. In fact, a human heart strain was more capable of entering a non-infectious, viable persistent stage when exposed to IFN-γ and was also more effectively rescued, compared to a human respiratory strain. Exquisite susceptibility to IFN-γ, specifically due to tryptophan availability appears to be a core adaptation of the human C. pneumoniae strains, which may reflect the chronic nature of their infections in this host.

Details

Title: Increased sensitivity to tryptophan bioavailability in a positive adaptation by the human strains of Chlamydia pneumoniae
Authors: Anu Chacko (Author) - Queensland University of Technology
Christopher J Barker (Author) - Queensland University of Technology
Kenneth W Beagley (Author) - Queensland University of Technology
Mark P Hodson (Author) - University of Queensland
Manuel R Plan (Author) - University of Queensland
Peter Timms (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Wilhelmina M Huston (Author) - Queensland University of Technology
Publication details: Molecular Microbiology, Vol.93(4), pp.797-813
Publisher: Wiley-Blackwell Publishing Ltd.
Date published: 2014
DOI: 10.1111/mmi.12701
ISSN: 0950-382X
Copyright note: Copyright © 2014 Wiley-Blackwell Publishing Ltd. This is the accepted version of the following article: Chacko, A., Barker, C. J., Beagley, K. W., Hodson, M. P., Plan, M. R., Timms, P. and Huston, W. M. (2014), Increased sensitivity to tryptophan bioavailability is a positive adaptation by the human strains of Chlamydia pneumoniae. Molecular Microbiology, 93: 797-813. doi: 10.1111/mmi.12701, which has been published in final form at http://dx.doi.org/10.1111/mmi.12701
Grants: Specificity of Chlamydia pneumoniae for humans: analysis of previous cross-host transmission events and the role of tryptophan availability in host specificity, 1004666, National Health and Medical Research Council (Australia, Canberra) - NHMRC
Organisation Unit: Centre for Bioinnovation
Language: English
Record Identifier: 99448829102621
Output Type: Journal article

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