Journal article
Increased allergic immune response to Sarcoptes scabiei antigens in crusted versus ordinary scabies
Clinical and Vaccine Immunology, Vol.17(9), pp.1428-1438
2010
Abstract
Scabies, a parasitic skin infestation by the burrowing 'itch' mite Sarcoptes scabiei, causes significant health problems for children and adults worldwide. Crusted scabies is a particularly severe form of scabies in which mites multiply in their millions, causing extensive skin crusting. The symptoms and signs of scabies suggest host immunity to the scabies mite, but the specific resistant response in humans remains largely uncharacterized. We used 4 scabies mite recombinant proteins with sequence homology to extensively studied house dust mite allergens to investigate a differential immune response between ordinary scabies and the debilitating crusted form of the disease. Subjects with either disease form showed serum IgE against recombinant S. scabiei cysteine and serine proteases and apolipoprotein, whereas naive subjects showed minimal IgE reactivity. Significantly (P < 0.05) greater serum IgE and IgG4 binding to mite apolipoprotein occurred in subjects with crusted scabies compared to those with ordinary scabies. Both subject groups showed strong proliferative responses (peripheral blood mononuclear cells) to the scabies antigens, but the crusted scabies group showed increased secretion of Th2 cytokines IL-5 and IL-13, and decreased Th1 cytokine IFN-gamma in response to the active cysteine protease. These data confirm that a non-protective allergic response occurs in the crusted disease form, and demonstrate that clinical severity is associated with differences in type and magnitude of the antibody and cellular responses to scabies proteins. Quantitative IgE inhibition assay identified IgE immunoreactivity of scabies mite antigens distinct from house dust mite antigens, of potential importance for specific scabies diagnosis and therapy.
Details
- Title
- Increased allergic immune response to Sarcoptes scabiei antigens in crusted versus ordinary scabies
- Authors
- Shelley F Walton (Author) - Charles Darwin UniversityS Pizzutto (Author) - Charles Darwin UniversityA Slender (Author) - Charles Darwin UniversityL Viberg (Author) - Charles Darwin UniversityD C Holt (Author) - Charles Darwin UniversityB J Hales (Author) - University of Western AustraliaD J Kemp (Author) - University of QueenslandB J Currie (Author) - Charles Darwin UniversityJ M Rolland (Author) - Monash UniversityR O'Hehir (Author) - Monash University
- Publication details
- Clinical and Vaccine Immunology, Vol.17(9), pp.1428-1438
- Publisher
- American Society for Microbiology
- Date published
- 2010
- DOI
- 10.1128/CVI.00195-10
- ISSN
- 1556-6811
- Copyright note
- Copyright © 2010 American Society for Microbiology. Reproduced here in accordance with the publishers copyright policy.
- Organisation Unit
- University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy
- Language
- English
- Record Identifier
- 99449663502621
- Output Type
- Journal article
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- Domestic collaboration
- Web Of Science research areas
- Immunology
- Infectious Diseases
- Microbiology
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