Increased TCP11 gene expression can inhibit the proliferation, migration and promote apoptosis of cervical cancer cells

Fang Wang; Shuyan Song; Bingxuan Guo; Yangyang Li; Huijuan Wang; Shaowei Fu; Luyue Wang; Xiangyi Zhe; Hongtao Li; Dongmei Li; Renfu Shao; Zemin Pan

doi:10.1186/s12885-023-11129-1

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Increased TCP11 gene expression can inhibit the proliferation, migration and promote apoptosis of cervical cancer cells

Journal article

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Increased TCP11 gene expression can inhibit the proliferation, migration and promote apoptosis of cervical cancer cells

Fang Wang, Shuyan Song, Bingxuan Guo, Yangyang Li, Huijuan Wang, Shaowei Fu, Luyue Wang, Xiangyi Zhe, Hongtao Li, Dongmei Li, …

BMC Cancer, Vol.23(1), pp.1-12

2023

DOI: https://doi.org/10.1186/s12885-023-11129-1

PMCID: PMC10496356

PMID: 37697257

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Abstract

Cervical cancer

TCP11 gene

Cell cycle

Apoptosis

Migration and proliferation

Background: Cervical cancer is a common gynecological malignancy. Gene microarray found that TCP11 gene was highly expressed in cervical cancer. However, the effect of TCP11 gene on the proliferation, apoptosis and migration of cervical cancer cells and its underlying molecular mechanisms are unclear. Methods: GEPIA database, tissue microarray, western blot and qRT-PCR were used to analyze the expression of TCP11 gene in cervical cancer tissues and cells and its relationship with patients' survival rate. The cell cycle and apoptosis were detected by flow cytometry, and the expressions of cell cycle and apoptosis related molecules and EMT-related molecules were detected by Western blot and qRT-PCR. Results: The results showed that TCP11 gene was highly expressed in cervical cancer tissues and cells compared with normal cervical tissues and cells, and its expression was positively correlated with patients' survival rate. The results of proliferation and migration assays showed that TCP11 overexpression inhibited the proliferation and migration of HeLa and SiHa cells. The results showed that TCP11 overexpression blocked the cell cycle of HeLa and SiHa cells, decreased the expression of CDK1 and Cyclin B1, and increased the apoptosis and the expression of caspase-3, cleaved-caspase-3 and cleaved-PARP. TCP11 overexpression increased the protein and mRNA expression of EMT-related molecules ZO-1 and E-cadherin. Conversely, TCP11 knockdown promoted the proliferation of HeLa and SiHa cells and the migration of HeLa cells. Conclusions: TCP11 overexpression significantly inhibited the occurrence and development of cervical cancer cells, it may be a potentially beneficial biomarker for cervical cancer.

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Title: Increased TCP11 gene expression can inhibit the proliferation, migration and promote apoptosis of cervical cancer cells
Authors: Fang Wang (Author) - Shihezi University
Shuyan Song (Author) - Shihezi University
Bingxuan Guo (Author) - Shihezi University
Yangyang Li (Author) - Shihezi University
Huijuan Wang (Author) - Shihezi University
Shaowei Fu (Author) - Shihezi University
Luyue Wang (Author) - Shihezi University
Xiangyi Zhe (Corresponding Author) - Shihezi University
Hongtao Li (Author) - Shihezi University
Dongmei Li (Author) - Shihezi University
Renfu Shao (Author) - University of the Sunshine Coast, Queensland, School of Science, Technology and Engineering
Zemin Pan (Corresponding Author) - Shihezi University
Publication details: BMC Cancer, Vol.23(1), pp.1-12
Publisher: BioMed Central Ltd.
Date published: 2023
DOI: 10.1186/s12885-023-11129-1
ISSN: 1471-2407
PMID: 37697257; PMC10496356
Copyright note: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
Grant note: 82060518, U1503125 / National Natural Science Foundation of China 2019BC007 / Xinjiang Production and Construction Corps
Organisation Unit: University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99966896302621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Oncology

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