Incorporation of fluorescein conjugated function-spacer-lipid constructs into the red blood cell membrane facilitates detection of labeled cells for the duration of ex-vivo storage

K K Ki; R L Flower; Helen M Faddy; Melinda M Dean

doi:10.1016/j.jim.2016.01.003

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Incorporation of fluorescein conjugated function-spacer-lipid constructs into the red blood cell membrane facilitates detection of labeled cells for the duration of ex-vivo storage

Journal article

Peer reviewed

Incorporation of fluorescein conjugated function-spacer-lipid constructs into the red blood cell membrane facilitates detection of labeled cells for the duration of ex-vivo storage

K K Ki, R L Flower, Helen M Faddy and Melinda M Dean

Journal of Immunological Methods, Vol.429, pp.66-70

2016

DOI: https://doi.org/10.1016/j.jim.2016.01.003

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Abstract

PRBC

ex-vivo storage

function-spacer-lipid

RBC labelling

The contribution of ex-vivo storage duration of packed red blood cells (PRBC) to patient outcomes and transfusion-related immunomodulation (TRIM) remains a broadly debated area in transfusion medicine. Kodeâ„¢ Technology with fluorescein conjugated function-spacer-lipid (FSL-FLRO4) constructs is a tool that can aid in-vitro visualization and tracking of red blood cells (RBC) during routine storage. FSL-FLRO4 is incorporated into the RBC membrane without altering cell function. In this study, we explore the suitability of this technology to label clinical grade PRBC and to determine if the label would be retained during ex-vivo storage. Firstly, to confirm feasibility and assess the limit of detection of FSL-FLRO4 on PRBC at date of expiry (42 days post-collection), we tracked the binding of FSL-FLRO4 on PRBC at weekly intervals during routine storage. Over the time course, all cells remained labelled with FSL-FLRO4, although a decrease in the intensity of labelling was observed (P < 0.0001). We then further investigated differences in FSL-FLRO4 labelling during RBC storage by labelling separated light-young and dense-old RBC from the same PRBC unit. There were no differences in the capacity of FSL-FLRO4 to label these different RBC subsets. Together, these data demonstrate that FSL-FLRO4 is a suitable reagent for labelling PRBC at any point during routine storage. This technology will facilitate the development of immunoassays and transfusion models focused on addressing the mechanisms involved in TRIM.

Details

Title: Incorporation of fluorescein conjugated function-spacer-lipid constructs into the red blood cell membrane facilitates detection of labeled cells for the duration of ex-vivo storage
Authors: K K Ki (Author) - Australian Red Cross Blood Service
R L Flower (Author) - Australian Red Cross Blood Service
Helen M Faddy (Author) - Australian Red Cross Blood Service
Melinda M Dean (Author) - Australian Red Cross Blood Service
Publication details: Journal of Immunological Methods, Vol.429, pp.66-70
Publisher: Elsevier BV
Date published: 2016
DOI: 10.1016/j.jim.2016.01.003
ISSN: 0022-1759; 0022-1759
Organisation Unit: School of Health - Biomedicine; University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; School of Health and Behavioural Sciences - Legacy
Language: English
Record Identifier: 99451438802621
Output Type: Journal article

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Collaboration types: Domestic collaboration
Web Of Science research areas: Biochemical Research Methods; Immunology

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