Inactivation of dengue, chikungunya, and Ross River viruses in platelet concentrates after treatment with ultraviolet C light

Helen M Faddy; J J Fryk; N A Prow; D Watterson; P R Young; R A Hall; F Tolksdorf; C Sumian; U Gravemann; A Seltsam; D C Marks

doi:10.1111/trf.13519

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Inactivation of dengue, chikungunya, and Ross River viruses in platelet concentrates after treatment with ultraviolet C light

Journal article

Peer reviewed

Inactivation of dengue, chikungunya, and Ross River viruses in platelet concentrates after treatment with ultraviolet C light

Helen M Faddy, J J Fryk, N A Prow, D Watterson, P R Young, R A Hall, F Tolksdorf, C Sumian, U Gravemann, A Seltsam, …

Transfusion, Vol.56(6, Part 2), pp.1548-1555

2016

DOI: https://doi.org/10.1111/trf.13519

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Abstract

Cardiovascular Medicine and Haematology

Clinical Sciences

Immunology

BACKGROUND: Arboviruses, including dengue (DENV 1-4), chikungunya (CHIKV), and Ross River (RRV), are emerging viruses that are a risk for transfusion safety globally. An approach for managing this risk is pathogen inactivation, such as the THERAFLEX UV-Platelets system. We investigated the ability of this system to inactivate the above mentioned arboviruses. STUDY DESIGN AND METHODS: DENV 1-4, CHIKV, or RRV were spiked into buffy coat (BC)-derived platelet (PLT) concentrates in additive solution and treated with the THERAFLEX UV-Platelets system at the following doses: 0.05, 0.1, 0.15, and 0.2 J/cm(2) (standard dose). Pre- and posttreatment samples were taken for each dose, and the level of viral infectivity was determined. RESULTS: At the standard ultraviolet C (UVC) dose (0.2 J/cm(2) ), viral inactivation of at least 4.43, 6.34, and 5.13 log or more, was observed for DENV 1-4, CHIKV, and RRV, respectively. A dose dependency in viral inactivation was observed with increasing UVC doses. CONCLUSIONS: Our study has shown that DENV, CHIKV, and RRV, spiked into BC-derived PLT concentrates, were inactivated by the THERAFLEX UV-Platelets system to the limit of detection of our assay, suggesting that this system could contribute to the safety of PLT concentrates with respect to these emerging arboviruses.

Details

Title: Inactivation of dengue, chikungunya, and Ross River viruses in platelet concentrates after treatment with ultraviolet C light
Authors: Helen M Faddy (Author) - University of Queensland
J J Fryk (Author)
N A Prow (Author)
D Watterson (Author)
P R Young (Author)
R A Hall (Author)
F Tolksdorf (Author)
C Sumian (Author)
U Gravemann (Author)
A Seltsam (Author)
D C Marks (Author)
Publication details: Transfusion, Vol.56(6, Part 2), pp.1548-1555
Publisher: Wiley-Blackwell Publishing Inc.
Date published: 2016
DOI: 10.1111/trf.13519
ISSN: 0041-1132
Organisation Unit: School of Health - Biomedicine; University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; School of Health and Behavioural Sciences - Legacy
Language: English
Record Identifier: 99450983902621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Hematology

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