Journal article
Inactivation of Japanese encephalitis virus in plasma by methylene blue combined with visible light and in platelet concentrates by ultraviolet C light
Transfusion, Vol.60(11), pp.2655-2660
2020
Abstract
Background: Japanese encephalitis virus (JEV) is endemic to tropical areas in Asia and the Western Pacific. It can cause fatal encephalitis, although most infected individuals are asymptomatic. JEV is mainly transmitted to humans through the bite of an infected mosquito, but can also be transmitted through blood transfusion. To manage the potential risk of transfusion transmission, pathogen inactivation (PI) technologies, such as THERAFLEX MB‐Plasma and THERAFLEX UV‐Platelets systems, have been developed. We examined the efficacy of these two PI systems to inactivate JEV. Study Design and Methods: Japanese encephalitis virus–spiked plasma units were treated using the THERAFLEX MB‐Plasma system (visible light doses, 20, 40, 60, and 120 [standard] J/cm2) in the presence of methylene blue at approximately 0.8 μmol/L and spiked platelet concentrates (PCs) were treated using the THERAFLEX UV‐Platelets system (UVC doses, 0.05, 0.10, 0.15, and 0.20 [standard] J/cm2). Samples were taken before the first and after each illumination dose and tested for infectivity using an immunoplaque assay. Results: Treatment of plasma with the THERAFLEX MB‐Plasma system resulted in an average of 6.59 log reduction in JEV infectivity at one‐sixth of the standard visible light dose (20 J/cm2). For PCs, treatment with the THERAFLEX UV‐Platelet system resulted in an average of 7.02 log reduction in JEV infectivity at the standard UVC dose (0.20 J/cm2). Conclusions: The THERAFLEX MB‐Plasma and THERAFLEX UV‐Platelets systems effectively inactivated JEV in plasma or PCs, and thus these PI technologies could be an effective option to reduce the risk of JEV transfusion transmission.
Details
- Title
- Inactivation of Japanese encephalitis virus in plasma by methylene blue combined with visible light and in platelet concentrates by ultraviolet C light
- Authors
- Lina Rustanti (Corresponding Author) - Australian Red Cross LifebloodJody Hobson-Peters (Author) - University of QueenslandA M G Colmant (Author) - University of QueenslandRoy Hall (Author) - University of QueenslandPaul R Young (Author) - University of QueenslandS Reichenberg (Author) - MacopharmaF Tolksdorf (Author) - MacopharmaC Sumian (Author) - MacopharmaU Gravemann (Author) - German Red CrossA Seltsam (Author) - German Red CrossD C Marks (Author) - Australian Red Cross LifebloodHelen Faddy (Author) - University of the Sunshine Coast, Queensland, School of Health and Sport Sciences - Legacy
- Publication details
- Transfusion, Vol.60(11), pp.2655-2660
- Publisher
- Wiley-Blackwell Publishing Inc.
- DOI
- 10.1111/trf.16021
- ISSN
- 1537-2995
- Organisation Unit
- School of Health and Behavioural Sciences - Legacy; School of Health and Sport Sciences - Legacy; School of Health - Biomedicine; University of the Sunshine Coast, Queensland
- Language
- English
- Record Identifier
- 99477803602621
- Output Type
- Journal article
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- Domestic collaboration
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- Hematology
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