Journal article
In vitro propagation of Corymbia torelliana × C. citriodora (Myrtaceae) via cytokinin-free node culture
Australian Journal of Botany, Vol.55(4), pp.471-481
2007
Abstract
Hybrids between Corymbia torelliana (F.Muell.) K.D.Hill & L.A.S.Johnson and C. citriodora subsp. variegata (F.Muell.) A.R.Bean & M.W.McDonald are used extensively to establish forestry plantations in subtropical Australia. Methods were developed for in vitro seed germination, shoot multiplication and plantlet formation that could be used to establish in vitro and ex vitro clone banks of juvenile Corymbia hybrids. Effects of sodium hypochlorite concentration and exposure time on seed contamination and germination, and effects of cytokinin and auxin concentrations on shoot multiplication and subsequent rooting, were assessed. A two-step surface sterilisation procedure, involving 70% ethanol followed by 1% sodium hypochlorite, provided almost no contamination and at least 88% germination. A novel method of cytokinin-free node culture proved most effective for in vitro propagation. Lateral bud break of primary shoots was difficult to induce by using cytokinin, but primary shoots rooted prolifically, elongated rapidly and produced multiple nodes in the absence of exogenous cytokinin. Further multiplication was obtained either by elongating lateral shoots of nodal explants in cytokinin-free medium or by inducing organogenic callus and axillary shoot proliferation with 2.2 µm benzyladenine. Plantlets were produced using an in vitro soil-less method that provided extensive rooting in sterile propagation mixture. These methods provide a means for simultaneous laboratory storage and field-testing of clones before selection and multiplication of desired genotypes.
Details
- Title
- In vitro propagation of Corymbia torelliana × C. citriodora (Myrtaceae) via cytokinin-free node culture
- Authors
- Stephen J Trueman (Author) - Queensland Department of Primary Industries and FisheriesD M Richardson (Author) - Queensland Department of Primary Industries and Fisheries
- Publication details
- Australian Journal of Botany, Vol.55(4), pp.471-481
- Publisher
- C S I R O Publishing
- Date published
- 2007
- DOI
- 10.1071/BT06163
- ISSN
- 0067-1924
- Organisation Unit
- School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy
- Language
- English
- Record Identifier
- 99448740302621
- Output Type
- Journal article
Metrics
2 File views/ downloads
818 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Web Of Science research areas
- Plant Sciences
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites