In vitro oocyte maturation by radial nerve extract and early development of the black sea cucumber (Holothuria Leucospilota)

Chieu Hoang Dinh; Saowaros Suwansa-ard; Tomer Abramov; Abigail Elizur; Scott F Cummins

doi:10.1016/j.aquaculture.2018.05.032

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In vitro oocyte maturation by radial nerve extract and early development of the black sea cucumber (Holothuria Leucospilota)

Journal article

Open access

Peer reviewed

In vitro oocyte maturation by radial nerve extract and early development of the black sea cucumber (Holothuria Leucospilota)

Chieu Hoang Dinh, Saowaros Suwansa-ard, Tomer Abramov, Abigail Elizur and Scott F Cummins

Aquaculture, Vol.495, pp.247-254

2018

DOI: https://doi.org/10.1016/j.aquaculture.2018.05.032

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Abstract

germinal vesicle breakdown

fertilization

maturation

radial nerve extract

sea cucumber

The popularity of sea cucumbers has led to overexploitation of wild populations and increasing need to develop their aquaculture. In vitro induction of oocyte maturation followed by fertilization represents an innovative approach that could help increase their production. The inducers of oocyte maturation are naturally synthesised within neural tissue, which in sea cucumbers includes longitudinal radial nerves and a buccal ring nerve. In this study, we showed that reproductive maturation of the black sea cucumber (Holothuria leucospilota Brandt, 1835) in South-East Queensland, Australia, peaked in December, based on biometric analysis of gonads. Radial nerve extract (RNE; 233 μg/ml) prepared from reproductive mature females induced in vitro oocyte maturation at 98.9% efficiency, based on germinal vesicle breakdown. RNE-matured oocytes were fertilized (99%) with conspecific sperm and larvae developed to late auricularia stage (25 days post-fertilization). The NGIWYamide neuropeptide (cubifrin), which induces oocyte maturation in the sea cucumber Apostichopus japonicus, could not induce oocyte maturation in H. leucospilota. These results provide information that could be used for large-scale artificial breeding of H. leucospilota, and a platform towards the identification of the bioactive RNE factor(s).

Details

Title: In vitro oocyte maturation by radial nerve extract and early development of the black sea cucumber (Holothuria Leucospilota)
Authors: Chieu Hoang Dinh (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Saowaros Suwansa-ard (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Tomer Abramov (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Abigail Elizur (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Scott F Cummins (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Publication details: Aquaculture, Vol.495, pp.247-254
Publisher: Elsevier BV
Date published: 2018
DOI: 10.1016/j.aquaculture.2018.05.032
ISSN: 0044-8486
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99450766802621
Output Type: Journal article

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Collaboration types: International collaboration
Web Of Science research areas: Fisheries; Marine & Freshwater Biology

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