Journal article
In vitro formation and growth of glycogen: experimental verification of theoretical predictions
Carbohydrate Polymers, Vol.368(Part 1), pp.1-8
2025
PMID: 40912811
Abstract
Glycogen is a complex branched glucose polymer that serves as energy reservoir in animals and some bacteria; it has also been synthesized in vitro. It comprises small β particles linked in large aggregates termed α particles. Theory, based on the evolutionary processes which cause these particles to be formed, suggests that if all ingredients for in vitro particle synthesis were added to a suspension of α particles, then these will grow to a steady-state size distribution, after which new particles will be formed. Here, this prediction is experimentally tested and found to be verified, using in vitro glycogen β particles as the starting point. The latter were made by the method of Ciric and Loos, Carbohydrate Polymers 2013, 93, 31; this was chosen because there are considerably less components than in an in vivo system, namely glycogen synthase, glycogen branching enzyme, glycogen debranching enzyme, glycogen phosphorylase and α-glucosidase. It was also found that new-particle formation was only found starting with β particles formed in vivo, but not with those synthesized in vitro; this implies that in vivo β particles contain one or more substances additional to those in the “minimal” in vitro particle synthesis used here.
Details
- Title
- In vitro formation and growth of glycogen: experimental verification of theoretical predictions
- Authors
- Xin Liu - Yangzhou UniversityZiyi Wang - Yangzhou UniversityXin Lin - The University of QueenslandWang Xu - The University of QueenslandMitchell A. Sullivan - University of the Sunshine CoastRobert G. Gilbert (Corresponding Author) - Yangzhou University
- Additional notes
- Corrigendum: The authors regret that Dr. Mitchell Sullivan was unintentionally omitted from the author list of the above-mentioned article at the time of publication.
- Publication details
- Carbohydrate Polymers, Vol.368(Part 1), pp.1-8
- Publisher
- Elsevier Ltd
- Date published
- 2025
- DOI
- 10.1016/j.carbpol.2025.124148
- ISSN
- 1879-1344
- PMID
- 40912811
- Data Availability
- Data will be made available on request.
- Grant note
- RGG gratefully acknowledges funding from the National Natural Science Foundation of China, grant number C1304013151101138, and the Priority Academic Program of Jiangsu Higher Education Institutions.
- Organisation Unit
- School of Health - Biomedicine; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 991222620402621
- Output Type
- Journal article
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- Chemistry, Applied
- Chemistry, Organic
- Polymer Science
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