Journal article
In vitro evaluation of combination of Trichoderma harzianum and chitosan for the control of sapstain fungi
Biological Control, Vol.50(3), pp.262-266
2009
Abstract
In vitro assays were undertaken to evaluate the control of two sapstain fungi, Leptographium procerum and Sphaeropsis sapinea by a combination of chitosan or chitosan oligomer and an albino strain of Trichoderma harzianum. Spore germination and hyphal growth of the test fungi were assessed on media amended with chitosan or chitosan oligomer with and without T. harzianum using either simultaneous inoculation with test fungus or 1, 2, or 3 days after pre-infection with test fungus.
There was no mycelial growth of the test fungi regardless of chitosan concentrations used when either L. procerum or S. sapinea was simultaneously inoculated with T. harzianum. However, the dose-response of chitosan or chitosan oligomer on the test fungi was apparent when T. harzianum was not simultaneously inoculated with test fungus but introduced later. There was a greater growth reduction at higher concentrations (0.075-0.1% v/v) of chitosan, and overall chitosan oligomer was more effective than chitosan aqueous solution.
Chitosan alone was able to restrict or delay the germination of spores but the combination of chitosan and T. harzianum inhibited spore germination and hence colony formation of test fungi regardless of time delay.
Details
- Title
- In vitro evaluation of combination of Trichoderma harzianum and chitosan for the control of sapstain fungi
- Authors
- Colleen Chittenden (Author) - ScionTripti Singh (Corresponding Author) - Scion
- Publication details
- Biological Control, Vol.50(3), pp.262-266
- Publisher
- Academic Press
- Date published
- 2009
- DOI
- 10.1016/j.biocontrol.2009.04.015
- ISSN
- 1090-2112
- Organisation Unit
- National Centre for Timber Durability and Design Life
- Language
- English
- Record Identifier
- 99979536202621
- Output Type
- Journal article
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- Web Of Science research areas
- Biotechnology & Applied Microbiology
- Entomology