In-vitro and in-vivo biological activity of recombinant Yellowtail kingfish (Seriola lalandi) follicle stimulating hormone

Pablo J Sanchis-Benlloch; Josephine N Nocillado; Claudia Ladisa; Joseph Aizen; Adam Miller; Michal Shpilman; Berta Levavi-Sivan; Tomer Ventura; Abigail Elizur

doi:10.1016/j.ygcen.2016.03.001

Back

In-vitro and in-vivo biological activity of recombinant Yellowtail kingfish (Seriola lalandi) follicle stimulating hormone

Journal article

Open access

Peer reviewed

In-vitro and in-vivo biological activity of recombinant Yellowtail kingfish (Seriola lalandi) follicle stimulating hormone

Pablo J Sanchis-Benlloch, Josephine N Nocillado, Claudia Ladisa, Joseph Aizen, Adam Miller, Michal Shpilman, Berta Levavi-Sivan, Tomer Ventura and Abigail Elizur

General and Comparative Endocrinology, Vol.241, pp.41-49

2017

DOI: https://doi.org/10.1016/j.ygcen.2016.03.001

Files and links (2)

pdf

PDF - Author's Accepted Version (Open Access)17.25 MBDownload View

Accepted VersionPDF - Author Accepted Version (Open Access)CC BY-NC-ND V4.0, Open Access

url

https://doi.org/10.1016/j.ygcen.2016.03.001View

Published Version

Abstract

recombinant protein

FSH

teleost puberty

Pichia pastoris

Seriola lalandi

Biologically active recombinant yellowtail kingfish follicle stimulating hormone (rytkFsh) was produced in yeast Pichia pastoris and its biological activity was demonstrated by both in-vitro and in-vivo bioassays. Incubation of ovarian and testicular fragments with the recombinant hormone stimulated E2 and 11-KT secretion, respectively. In-vivo trial in immature female YTK resulted in a significant increase of plasma E2 levels and development of oocytes. In males at the early stages of puberty, advancement of spermatogenesis was observed, however plasma 11-KT levels were reduced when administered with rytkFsh.

Details

Title: In-vitro and in-vivo biological activity of recombinant Yellowtail kingfish (Seriola lalandi) follicle stimulating hormone
Authors: Pablo J Sanchis-Benlloch (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Josephine N Nocillado (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Claudia Ladisa (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Joseph Aizen (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Adam Miller (Author) - Clean Seas Tuna Ltd.
Michal Shpilman (Author) - Hebrew University of Jerusalem, Israel
Berta Levavi-Sivan (Author) - Hebrew University of Jerusalem, Israel
Tomer Ventura (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Abigail Elizur (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Publication details: General and Comparative Endocrinology, Vol.241, pp.41-49
Publisher: Academic Press
Date published: 2017
DOI: 10.1016/j.ygcen.2016.03.001
ISSN: 0016-6480
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99449918802621
Output Type: Journal article

Metrics

110 File views/ downloads

2003 Record Views

47 Times Cited - Web of Science

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Endocrinology & Metabolism; Zoology

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

Source: InCites