In vitro analysis of genetically distinct Chlamydia pecorum isolates reveals key growth differences in mammalian epithelial and immune cells

Md Mominul Islam; Martina Jelocnik; Susan Anstey; Bernhard Kaltenboeck; Nicole Borel; Peter Timms; Adam Polkinghorne

doi:10.1016/j.vetmic.2019.03.024

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In vitro analysis of genetically distinct Chlamydia pecorum isolates reveals key growth differences in mammalian epithelial and immune cells

Journal article

Peer reviewed

In vitro analysis of genetically distinct Chlamydia pecorum isolates reveals key growth differences in mammalian epithelial and immune cells

Md Mominul Islam, Martina Jelocnik, Susan Anstey, Bernhard Kaltenboeck, Nicole Borel, Peter Timms and Adam Polkinghorne

Veterinary Microbiology, Vol.232, pp.22-29

2019

DOI: https://doi.org/10.1016/j.vetmic.2019.03.024

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Abstract

Chlamydia pecorum

In vitro growth

Genetically distinct

Developmental cycle

Chlamydia (C.) pecorum is an obligate intracellular bacterium that infects and causes disease in a broad range of animal hosts. Molecular studies have revealed that this pathogen is genetically diverse with certain isolates linked to different disease outcomes. Limited in vitro or in vivo data exist to support these observations, further hampering efforts to improve our understanding of C. pecorum pathogenesis. In this study, we evaluated whether genetically distinct C. pecorum isolates (IPA, E58, 1710S, W73, JP-1-751) display different in vitro growth phenotypes in different mammalian epithelial and immune cells. In McCoy cells, shorter lag phases were observed for W73 and JP-1-751 isolates. Significantly smaller inclusions were observed for the naturally plasmid-free E58 isolate. C. pecorum isolates of bovine (E58) and ovine origin (IPA, W73, JP-1-751) grew faster in bovine cells compared to a porcine isolate (1710S). C. pecorum isolates could infect but appear not able to complete their developmental cycle in bovine peripheral neutrophil granulocytes. All isolates, except 1710S, could multiply in bovine monocyte-derived macrophages. These results reveal potentially important phenotypic differences that will help to understand the pathogenesis of C. pecorum in vivo and to identify C. pecorum virulence factors.

Details

Title: In vitro analysis of genetically distinct Chlamydia pecorum isolates reveals key growth differences in mammalian epithelial and immune cells
Authors: Md Mominul Islam (Corresponding Author) - University of the Sunshine Coast
Martina Jelocnik (Author) - University of the Sunshine Coast
Susan Anstey (Author) - University of the Sunshine Coast
Bernhard Kaltenboeck (Author) - Auburn University, United States
Nicole Borel (Author) - University of Zurich, Switzerland
Peter Timms (Author) - University of the Sunshine Coast
Adam Polkinghorne (Author) - University of the Sunshine Coast
Publication details: Veterinary Microbiology, Vol.232, pp.22-29
Publisher: Elsevier BV
Date published: 2019
DOI: 10.1016/j.vetmic.2019.03.024
ISSN: 0378-1135; 1873-2542
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99451118102621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Microbiology; Veterinary Sciences

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